Analytical Data
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基因名
IL-17F
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简介
Rat IL-17F is predicted to have cytokine binding and dimerization activities and is involved in bacterial defense, IL-17-mediated signaling, and regulation of cytokine production. It acts upstream of the inflammatory response, and its homologue to human IL17F has been implicated in chronic mucocutaneous candidiasis. IL-17F Protein, Rat (sf9, N-His) is the recombinant rat-derived IL-17F, Rat, expressed by E. coli , with N-6*His labeled tag.
- Application
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生物活性
Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 45.19 ng/mL, corresponding to a specific activity is 2.212×104 U/mg. Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 45.19 ng/mL, corresponding to a specific activity is 2.212×104 U/mg.
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别名
Cytokine ML-1; IL17F; Interleukin-17F
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种属
Rat
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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蛋白编号
NP_001015011.2
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表达区间
A28-A161
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蛋白长度
Full Length of Mature Protein
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分子量
18 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-17F (IL-17F) is a potent pro-inflammatory cytokine that belongs to the IL-17 family, which includes several members known for their pivotal roles in autoimmune diseases and inflammatory responses. Research on IL-17F has gained significant attention due to its involvement in various conditions, such as asthma, psoriasis, and autoimmune diseases like rheumatoid arthritis. Unlike its closely related counterpart IL-17A, IL-17F exhibits unique biological activities and signaling pathways that contribute to tissue inflammation and remodeling. Characterization of the IL-17F protein has advanced significantly, with recombinant forms of IL-17F being produced for therapeutic and research purposes. Understanding the structure-function relationship of IL-17F provides insights into the mechanism of action and potential therapeutic targets for blocking its activity. Studies have demonstrated that neutralizing IL-17F can mitigate inflammation and improve disease outcomes in preclinical models, highlighting its potential as a therapeutic target. Furthermore, ongoing research aims to elucidate the interactions between IL-17F and other cytokines in the immune response, as well as its potential role in skewing T-cell differentiation. Overall, the investigation of IL-17F and its recombinant proteins is crucial for developing novel immunomodulatory therapies aimed at treating inflammatory diseases more effectively.
