Analytical Data
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Gene name
IL-25
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简介
IL-25/IL-17E cytokines are produced by eosinophils, Th2 cells, and epithelial cells and critically regulate the adaptive immune response by modulating cytokine production. It enhances local and systemic type 2 helper T cell responses through its IL17RA and IL17RB receptors and activates the JAK2-STAT5A pathway. IL-25/IL-17E Protein, Human (HEK293, Fc) is the recombinant human-derived IL-25/IL-17E protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Alternative Names
Interleukin-25; IL-25; Interleukin-17E; IL-17E
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Species
Human
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Source
HEK293
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Tag
N-hFc
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Purity
> 90% as determined by SDS-PAGE.
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Uniprot
Q9H293
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Expression Region
Y33-G177
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
50-60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-25 (IL-25), also known as IL-17E, is a member of the IL-17 cytokine family that plays a crucial role in immune responses, particularly in promoting type 2 immunity. It is primarily produced by epithelial cells and plays a significant role in regulating the function of various immune cells, including T helper 2 (Th2) cells, mast cells, and eosinophils. The research surrounding IL-25 has gained momentum due to its involvement in allergic reactions, asthma, and other inflammatory diseases. Recent studies suggest that IL-25 can enhance the production of other cytokines, such as IL-4, IL-5, and IL-13, which are pivotal in driving Th2-mediated immune responses. Given its role in these pathways, IL-25 has emerged as a potential therapeutic target for treating allergic diseases and autoimmune disorders. The development of recombinant IL-25 proteins allows scientists to explore its biological functions and investigate its potential applications in immunotherapy and vaccine development. This research not only helps in understanding the pathogenic mechanisms of various diseases but also paves the way for novel treatment strategies that could modulate immune responses by targeting IL-25 signaling pathways.
