Analytical Data
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Gene name
IL-25
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简介
Interleukin 25, also known as IL-17E, is a unique cytokine of the IL-17 family. Interleukin 25 exclusively was shown to strongly induce expression of the cytokines associated with type 2 immunity. Interleukin 25 is a “barrier surface” cytokine whose expression depends on extrinsic environmental factors and when up-regulated may lead to inflammatory disorders such as atopic dermatitis, psoriasis or asthma. IL-25/IL-17E Protein, Canine (HEK293, Fc) is the recombinant canine-derived IL-25/IL-17E protein, expressed by HEK293 , with N-hFc labeled tag.
- Application
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Biological Activity
Measured by its ability to induce CXCL1 secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 1.361 ng/mL, corresponding to a specific activity is 7.348×105 U/mg. Measured by its ability to induce CXCL1 secretion in HT‑29 human colon adenocarcinoma cells. The ED50 for this effect is 1.361 ng/ml, corresponding to a specific activity is 7.348×105 U/mg.
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Alternative Names
Interleukin-25; IL-25; Interleukin-17E; IL-17E
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Species
Canine
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Source
HEK293
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Tag
N-hFc
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
A0A8I3MRV2
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Expression Region
L17-A169
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Protein Length
Partial
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Molecular Weight
50-55 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-25 (IL-25), also known as IL-17E, is a member of the IL-17 cytokine family that plays a crucial role in immune responses, particularly in promoting type 2 immunity. It is primarily produced by epithelial cells and plays a significant role in regulating the function of various immune cells, including T helper 2 (Th2) cells, mast cells, and eosinophils. The research surrounding IL-25 has gained momentum due to its involvement in allergic reactions, asthma, and other inflammatory diseases. Recent studies suggest that IL-25 can enhance the production of other cytokines, such as IL-4, IL-5, and IL-13, which are pivotal in driving Th2-mediated immune responses. Given its role in these pathways, IL-25 has emerged as a potential therapeutic target for treating allergic diseases and autoimmune disorders. The development of recombinant IL-25 proteins allows scientists to explore its biological functions and investigate its potential applications in immunotherapy and vaccine development. This research not only helps in understanding the pathogenic mechanisms of various diseases but also paves the way for novel treatment strategies that could modulate immune responses by targeting IL-25 signaling pathways.
