Analytical Data
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Gene name
CD146/MCAM
- Application
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Alternative Names
MCAM; MUC18; Melanoma Cell Adhesion Molecule; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by reducing SDS-PAGE.
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Uniprot
P43121
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Expression Region
Asp324~Val565
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Molecular Weight
33kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD146, also known as MCAM (Melanoma Cell Adhesion Molecule), is a transmembrane glycoprotein primarily expressed on endothelial cells and various types of immune cells. It plays a crucial role in cell adhesion, migration, and communication within the tumor microenvironment, making it a significant focus of cancer research. Elevated expression levels of CD146/MCAM have been linked to tumor progression, angiogenesis, and metastasis in several malignancies, including melanoma, breast cancer, and glioblastoma. As a research tool, recombinant CD146/MCAM proteins are essential for elucidating the molecule's functional roles and mechanisms in cellular interactions and signaling pathways. Their application in in vitro studies allows for the investigation of the adhesion properties and migratory behavior of cancer cells, potentially unveiling novel therapeutic targets. Additionally, recombinant forms can be utilized in the development of diagnostic assays and therapeutic antibodies, advancing personalized medicine approaches. Understanding the biology of CD146/MCAM is vital not only for deciphering its contributions to tumor biology but also for exploring its potential as a biomarker and therapeutic target in cancer treatment.
