Analytical Data
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基因名
CD146/MCAM
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简介
The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL. Measured by its binding ability in a functional ELISA. When Recombinant Mouse MCAM/CD146 is immobilized at 1 μg/mL, the concentration of Recombinant Mouse Galectin-3 that produces 50% of the optimal binding response is 0.4829 μg/mL.
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别名
Cell surface glycoprotein MUC18; Gicerin; CD146; Muc18
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种属
Mouse
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
Q8R2Y2
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表达区间
V24-V563
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氨基酸序列
VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVV
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蛋白长度
Extracellular Domain
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分子量
90-100 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
CD146, also known as MCAM (Melanoma Cell Adhesion Molecule), is a transmembrane glycoprotein primarily expressed on endothelial cells and various types of immune cells. It plays a crucial role in cell adhesion, migration, and communication within the tumor microenvironment, making it a significant focus of cancer research. Elevated expression levels of CD146/MCAM have been linked to tumor progression, angiogenesis, and metastasis in several malignancies, including melanoma, breast cancer, and glioblastoma. As a research tool, recombinant CD146/MCAM proteins are essential for elucidating the molecule's functional roles and mechanisms in cellular interactions and signaling pathways. Their application in in vitro studies allows for the investigation of the adhesion properties and migratory behavior of cancer cells, potentially unveiling novel therapeutic targets. Additionally, recombinant forms can be utilized in the development of diagnostic assays and therapeutic antibodies, advancing personalized medicine approaches. Understanding the biology of CD146/MCAM is vital not only for deciphering its contributions to tumor biology but also for exploring its potential as a biomarker and therapeutic target in cancer treatment.
