Analytical Data
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Gene name
Cadherin-1/CD324
- Application
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Biological Activity
Measured by its ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 is 0.5617 μg/mL, corresponding to a specific activity of 1.78 × 103 units/mg. Measured by its ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 is 0.5617 μg/mL, corresponding to a specific activity of 1.78 × 103 units/mg.
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Alternative Names
rHuCadherin-1/CD324, His; Cadherin-1; CDH1; CAM 120/80; E-cadherin; CD324
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P12830-1
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Expression Region
D155-I707
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AA Sequence
DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIHHHHHH
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Protein Length
Extracellular Domain
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Molecular Weight
80-90 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Cadherin-1, also known as CD324, is a classical type of cadherin that plays a crucial role in cell-cell adhesion, tissue morphogenesis, and development. It is primarily expressed in epithelial tissues and is fundamental for maintaining the integrity and stability of epithelial structures. The study of recombinant Cadherin-1 is significant due to its involvement in various biological processes, including cell signaling, differentiation, and tumor progression. Aberrant expression of Cadherin-1 has been associated with several cancers, particularly in the context of epithelial-to-mesenchymal transition (EMT), which is a key process in cancer metastasis. The creation of recombinant Cadherin-1 proteins allows researchers to investigate its structural properties, binding mechanisms, and functional roles in cellular contexts. These studies can pave the way for developing therapeutic strategies targeting cadherin-mediated pathways. Additionally, understanding the molecular basis of Cadherin-1's function could provide insights into developmental biology and the potential for novel interventions in cancer treatment, highlighting its importance in both basic and applied biomedical research.
