Analytical Data
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Gene name
JAM-A/CD321
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简介
The JAM-A/CD321 protein is critical for the formation of epithelial tight junctions and is present at primitive cell junctions where it recruits PARD3.This association may hinder PARD3-JAM1 interaction and thus tight junction assembly.JAM-A/CD321 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived JAM-A/CD321 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
Junctional Adhesion Molecule A; JAM-A; JAM-1; PAM-1; CD321; F11R; JCAM
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Species
Mouse
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O88792
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Expression Region
K27-A242
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Protein Length
Partial
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Molecular Weight
57 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
JAM-A (Junctional Adhesion Molecule A), also known as CD321, is a member of the immunoglobulin superfamily and plays a crucial role in cell-cell adhesion, particularly in endothelial and epithelial cells. It is predominantly expressed at the tight junctions of these cells, influencing various physiological processes, including leukocyte transmigration, barrier function, and tissue homeostasis. Its interaction with other adhesion molecules, such as integrins and claudins, highlights its importance in maintaining the integrity of the vascular system and the immune response. Research has demonstrated that JAM-A is involved in several pathological conditions, including inflammation, cancer metastasis, and autoimmune diseases, making it a significant target for therapeutic intervention. The study of JAM-A/CD321 recombinant protein offers insights into its structure and functional mechanisms, providing a basis for developing drugs aimed at modulating its activity. Understanding the role of this protein in cellular signaling and adhesion can lead to novel strategies for treating diseases associated with compromised endothelial barriers or dysregulated immune responses. Furthermore, the recombinant protein serves as a valuable tool for probing the molecular pathways influenced by JAM-A, establishing its potential as a biomarker for various diseases and as a target for biomolecular therapy.
