Analytical Data
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基因名
JAM-A/CD321
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简介
The JAM-A/CD321 protein is critical for the formation of tight junctions in epithelial cells, participating in early junction development and recruiting PARD3. However, the formation of PARD6-PARD3 complex may hinder PARD3-JAM1 interaction, thus preventing tight junction assembly. JAM-A/CD321 Protein, Rat (HEK293, His) is the recombinant rat-derived JAM-A/CD321 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by the ability of the immobilized protein to inhibit the adhesion of Vitronectin on HUVEC cells. The ED50 for this effect is 0.1574 μg/mL in the presence of 10 ng/mL Vitronectin. Corresponding to a specific activity is 6353.24 U/mg. Measured by the ability of the immobilized protein to inhibit the adhesion of Vitronectin on HUVEC cells. The ED50 for this effect is 0.1574 μg/mL in the presence of 10 ng/mL Vitronectin. Corresponding to a specific activity is 6353.24 U/mg.
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别名
Junctional Adhesion Molecule A; JAM-A; JAM-1; PAM-1; CD321; F11R; JCAM
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种属
Rat
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
Q9JHY1
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表达区间
K27-G238
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氨基酸序列
KGSVYSPQTAVQVPENDSVKLPCIYSGFSSPRVEWKFVQGSTTALVCYNNQITVPYADRVTFSSSGITFSSVTRKDNGEYTCMVSEDGGQNYGEVSIHLTVLVPPSKPTVSIPSSVTIGNRAVLTCSEHDGSPPSEYSWFKDGVPMLTADAKKTRAFINSSYTIDPKSGDLVFDPVSAFDSGEYYCEAQNGYGTAMRSEAVRMEAVELNVGG
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蛋白长度
Extracellular Domain
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分子量
25&28 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
JAM-A (Junctional Adhesion Molecule A), also known as CD321, is a member of the immunoglobulin superfamily and plays a crucial role in cell-cell adhesion, particularly in endothelial and epithelial cells. It is predominantly expressed at the tight junctions of these cells, influencing various physiological processes, including leukocyte transmigration, barrier function, and tissue homeostasis. Its interaction with other adhesion molecules, such as integrins and claudins, highlights its importance in maintaining the integrity of the vascular system and the immune response. Research has demonstrated that JAM-A is involved in several pathological conditions, including inflammation, cancer metastasis, and autoimmune diseases, making it a significant target for therapeutic intervention. The study of JAM-A/CD321 recombinant protein offers insights into its structure and functional mechanisms, providing a basis for developing drugs aimed at modulating its activity. Understanding the role of this protein in cellular signaling and adhesion can lead to novel strategies for treating diseases associated with compromised endothelial barriers or dysregulated immune responses. Furthermore, the recombinant protein serves as a valuable tool for probing the molecular pathways influenced by JAM-A, establishing its potential as a biomarker for various diseases and as a target for biomolecular therapy.
