Analytical Data
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Gene name
ICAM-1/CD54
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简介
The ICAM-1/CD54 protein is a ligand of the leukocyte adhesion protein LFA-1 and is critical for leukocyte transendothelial migration. Its involvement activates ARHGEF26/SGEF and RHOG, coordinating endothelial apical cup assembly. ICAM-1/CD54 Protein, Rhesus Macaque (HEK293, Fc) is the recombinant Rhesus Macaque-derived ICAM-1/CD54 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ITGAL&ITGB2 Heterodimer is immobilized at 10 µg/mL (100 µL/well) can bind Biotinylated Rhesus Macaque ICAM-1. The ED50 for this effect is 1.539 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ITGAL&ITGB2 Heterodimer is immobilized at 10 µg/mL (100 µL/well) can bind Biotinylated Rhesus Macaque ICAM-1. The ED50 for this effect is 1.539 ng/mL.
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Alternative Names
Intercellular Adhesion Molecule 1; ICAM-1; Major Group Rhinovirus Receptor; CD54; ICAM1
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Species
Rhesus Macaque
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5NKV6
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Expression Region
Q28-E480
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AA Sequence
QTSVFPPEVILPRGGSVKVNCSASCDQPISLGMETPLPKKEILPGGNNWKMYELSNVQEDSQPMCYSNCPDGQSSAKTLLTVYWTPERVELAPLPPWQPVGKNLTLRCQVEGGAPRANLTVMLLRGEKELSRQSAVGEPAEVTTTVPVGRDDHGANFSCRTELDLRPYVLKLFENTSAPHQLQTFDLPATPPQLVSPQVLEVDTQGTVVCSLDGLFPVSEAQVSLALGDQKLNPTITYGNNSLSAKASVKVTAEEEGTQQLLCGVMLGNQTQETRQTVTIYSFPAPNVNLTKPEVSEGTEVIVECEAHPRAKVMLNGVPAQPPGPRAQFLLKATPEDNGRSFSCSATLEVAGQLVHKNQTRELRVLYGPRLDEKDCPGNWTWPENSQQTPMCQAWGNPLPQLKCLKDGTFPLPIGQSVTVTRDLEGTYLCQARSTRGEVTREVTVNVLSPRYE
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Protein Length
Extracellular Domain
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Molecular Weight
100-130 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ICAM-1 (Intercellular Adhesion Molecule-1), also known as CD54, is a crucial adhesion molecule expressed on endothelial cells and immune cells, playing a significant role in inflammation and immune response. Its primary function is to facilitate the binding of leukocytes to endothelial cells, which is critical during immune responses and the migration of immune cells to sites of inflammation. Dysregulation of ICAM-1 can result in various pathological conditions, including chronic inflammation, autoimmune diseases, and tumor progression. The study of recombinant ICAM-1/CD54 proteins has gained attention in recent years due to their potential applications in therapeutic interventions and vaccine development. Recombinant forms of this protein allow researchers to investigate its structural and functional properties in detail, providing insights into its role in immune mechanisms. Moreover, understanding how ICAM-1/CD54 interacts with other molecules, such as ligands on lymphocytes, can pave the way for novel therapeutic strategies aimed at modulating immune responses. These recombinant proteins are also valuable tools for studying the molecular basis of adhesion and signaling processes involved in immune cell activation. Overall, the research on ICAM-1/CD54 recombinant proteins is significant in elucidating the complexities of immune interactions and may lead to innovative approaches for treating inflammatory diseases and enhancing vaccine efficacy.
