Analytical Data
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基因名
IL-2
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简介
IL-2 protein is mainly produced by activated CD4-positive helper T cells and plays an important role in immune response and tolerance. Binding to IL-2R induces downstream signaling through JAK1 and JAK3 phosphorylation, thereby activating various pathways, including STAT, PI3K, and MAPK. IL-2 Protein, Mouse is the recombinant mouse-derived IL-2 protein, expressed by E. coli.
- Application
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生物活性
1.Measured in a cell proliferation assay using CTLL-2 Cells. The ED50 for this effect is ≤0.5117 ng/mL, corresponding to a specific activity is 1.954×106 units/mg. 2.Measured in a cell proliferation assay using CTLL 2 mouse cytotoxic T cells.The specific activity of Recombinant Mouse IL-2 is ≥1×106 IU/mg. Measured in a cell proliferation assay using CTLL-2 Cells. The ED50 for this effect is 0.5117 ng/mL, corresponding to a specific activity is 1.954×106 units/mg.
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别名
rMuIL-2; IL2; T-cell Growth Factor; TCGF; Aldesleukin
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种属
Mouse
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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蛋白编号
P04351
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表达区间
A21-Q169
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蛋白长度
Full Length of Mature Protein
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分子量
17 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-2 (IL-2) is a pivotal cytokine in the immune system, primarily produced by T cells, and it plays a crucial role in the proliferation and activation of immune cells, particularly T lymphocytes. The recombinant form of IL-2 has gained significant attention in biomedical research and therapeutic applications due to its potential to enhance immune responses. Originally discovered for its capacity to support T cell growth, IL-2 has been researched extensively for its implications in cancer immunotherapy, autoimmune diseases, and transplant acceptance. The ability to produce IL-2 recombinantly has enabled scientists to explore its effects in a controlled manner, paving the way for the development of novel treatments aimed at boosting anti-tumor immunity or modulating excessive immune responses. Research involving IL-2 recombinant protein has led to a better understanding of its mechanisms, such as promoting T cell differentiation and memory formation, while also identifying optimal dosing strategies and delivery methods to maximize efficacy and minimize adverse effects. Furthermore, recombinant IL-2 has been integral in developing adoptive cell therapies, such as CAR T-cell therapy, where it is used to enhance the survival and functionality of engineered T cells. Overall, the study of IL-2 recombinant protein represents a cornerstone of immunotherapy research, highlighting its versatility and importance in manipulating the immune system for therapeutic benefit.
