Analytical Data
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基因名
CD58
- Application
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生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized human CD2-His at 10 μg/mL (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.1 μg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD2-His at 10 μg/mL (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.10 μg/mL. 3.Loaded Recombinant Human CD58/LFA-3 Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human CD2 Protein, His Tag with an affinity constant of 1.36 μM as determined in BLI assay.
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别名
Lymphocyte Function-Associated Antigen 3; LFA3; Ag3; CD58 antigen
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种属
Human
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BRW0
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表达区间
F29-R215
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蛋白长度
Extracellular Domain
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分子量
68 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a cell surface protein that plays a critical role in the immune system by facilitating the interaction between antigen-presenting cells and T cells. Understanding CD58's structure and function is vital for developing targeted immunotherapies for various diseases, including cancers and autoimmune disorders. The recombinant form of CD58 has been utilized in various studies to elucidate its effects on T-cell activation and proliferation. Research indicates that CD58 enhances T-cell responses by binding to CD2, promoting stronger adhesion and signaling pathways necessary for effective immune responses. Recent investigations focus on the therapeutic potential of CD58 in enhancing the efficacy of existing immunotherapies, as well as its role in the tumor microenvironment where it may influence immune evasion mechanisms employed by cancer cells. The generation of CD58 recombinant protein allows for detailed studies into its mechanistic roles, interactions, and potential as a biomarker in clinical settings. This research could pave the way for novel treatments that harness the body's immune system more effectively, emphasizing the importance of CD58 in immune modulation.
