Analytical Data
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基因名
E-Selectin/CD62E
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简介
The E-selectin/CD62E protein belongs to the selectin/LECAM family and plays a crucial role in mediating cell adhesion processes, particularly the interaction between leukocytes and endothelial cells. As a member of this family, E-selectin may have conserved characteristics, underscoring its importance in cell adhesion. E-selectin/CD62E Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived E-selectin/CD62E protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 29.560 ng/mL, corresponding to a specific activity is 3.392×104 units/mg. Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. The ED50 for this effect is 29.560 ng/mL, corresponding to a specific activity is 3.392×104 units/mg.
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别名
E-selectin; ELAM-1; LECAM2; CD63E; SELE
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种属
Cynomolgus
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
G8F370
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表达区间
W22-P556
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氨基酸序列
WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKRDKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLECEQIVNCTALESPEHGSLVCSHPLGNFSYSSSCSVSCDRGYLPSSVETTQCMSSGEWSVPIPACKVVECDAVTNPANGFVECFQNPGSFPWNTTCTFDCEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAIRQPQNGSVRCSHSPAGEFTFKSSCNFTCEEGFMLQGAAQVECTTQGQWTQQVPVCEAFQCTALSNPERGYMNCLPSASGSFRNGSSCEFSCEQGFVLKGSKRLQCGPTGEWDNEKPTCEAVRCDAVHQPQRGLVRCAHSPIGEFTYKSSCAFSCEEGFELHGSTQLECTSQGQWTEEVPSCQVVKCSSLAVLEKINMSCSGEPVFGTVCNFACPEGWRLNGSAAMTCGATGHWSGMLPTCEAPTESNTP
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蛋白长度
Partial
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分子量
95-115 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
E-Selectin, also known as CD62E, is a cell adhesion molecule that plays a crucial role in the inflammatory response and leukocyte trafficking. It is primarily expressed on endothelial cells activated by pro-inflammatory cytokines, facilitating the recruitment of leukocytes to sites of inflammation. Research into E-Selectin has gained significant attention due to its involvement in various pathophysiological conditions, including cardiovascular diseases, cancer, and chronic inflammatory disorders. The recombinant form of E-Selectin, generated through modern biotechnological methods, allows for detailed studies of its structure, function, and interactions with ligands such as sialyl Lewisx (sLex) carbohydrate, which is crucial for leukocyte rolling and adhesion. By producing and characterizing E-Selectin recombinant proteins, researchers aim to gain insights into its exact role in immune responses, potential therapeutic applications, and the development of anti-inflammatory strategies. Additionally, the study of E-Selectin can aid in the design of novel drug delivery systems and biomaterials that target inflammatory sites, making it a promising area of research in immunology and therapeutic interventions. Overall, the investigation of E-Selectin/CD62E recombinant proteins represents an important aspect of understanding and manipulating immune cell interactions, with broad implications for treating diseases characterized by dysregulated inflammation.
