Analytical Data
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基因名
TLR4
- Application
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别名
TLR4；Toll-like receptor 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O00206
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表达区间
326-634aa
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氨基酸序列
FSYNFGWQHLELVNCKFGQFPTLKLKSLKRLTFTSNKGGNAFSEVDLPSLEFLD LSRNGLSFKGCCSQSDFGTTSLKYLDLSFNGVITMSSNFLGLEQLEHLDFQHSN LKQMSEFSVFLSLRNLIYLDISHTHTRVAFNGIFNGLSSLEVLKMAGNSFQENF LPDIFTELRNLTFLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYK CLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIK DQRQLLVEVERMECATPSDKQGMPVLSLNITCQMNKTII
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分子量
42.0kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
Toll-like receptor 4 (TLR4) is a crucial component of the innate immune system, playing a significant role in recognizing pathogen-associated molecular patterns (PAMPs) and initiating immune responses. The study of TLR4 and its recombinant protein has garnered considerable attention due to its involvement in various infectious and inflammatory diseases. TLR4 recognizes lipopolysaccharides (LPS) from Gram-negative bacteria, leading to the activation of downstream signaling pathways that elevate the expression of pro-inflammatory cytokines, chemokines, and other immune mediators. Dysregulation of TLR4 signaling is associated with numerous pathological conditions, including sepsis, autoimmune disorders, and chronic inflammation. Recombinant TLR4 proteins have emerged as valuable tools for research, allowing scientists to explore its structure-function relationship, ligand interactions, and signaling mechanisms in a controlled environment. Furthermore, the generation of TLR4 recombinant proteins facilitates the development of therapeutic strategies aimed at modulating TLR4 activity, either by enhancing its function to boost immune responses or by inhibiting it in conditions of excessive inflammation. This dual potential positions TLR4 as a key target for new interventions in treating infections and inflammatory diseases, thereby highlighting the importance of continued research into its recombinant forms.
