Analytical Data
-
基因名
rnfH
- Application
-
别名
rnfH；Protein RnfH
-
种属
Coxiella
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
B6IZH9
-
表达区间
1-101aa
-
氨基酸序列
MISIIIAYATPEKQVEIPLTVEESCTLVVAVKRSGILQQFPEINLSQAIVGIHNKRTALDAGLRDGDRIEIYRPLTMDPKQARLLRAKRGKIRRMVRGEAG
-
分子量
27.3 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The rnfH gene, known to encode a Na+-translocating ferredoxin:NADP+ oxidoreductase, has garnered significant interest due to its role in energy metabolism in various microorganisms, particularly in anaerobic environments. This enzyme is crucial for the biological conversion of energy from electron donors to applicable forms for cellular processes. The study of recombinant rnfH proteins has grown in relevance as researchers seek to elucidate the mechanistic details of Na+ transport and its coupling to redox reactions, which are fundamental to bioenergetics. Investigating rnfH recombinants can provide insights into the evolution of electron transport systems, highlight potential biotechnological applications, and improve our understanding of microbial physiology under different environmental conditions. Furthermore, characterizing the recombinant protein's structure and function can pave the way for innovative approaches in metabolic engineering, bioenergy production, and bioremediation strategies, contributing to sustainable development. Overall, the exploration of rnfH and its recombinant variants stands at the intersection of microbiology, biochemistry, and applied sciences, making it a vital area of study.
