Cat: PA2000-7977

Recombinant Human GLDN Protein,His

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Analytical Data

  • Gene name

    GLDN

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    GLDN; COLM; UNQ9339/PRO34011Gliomedin [Cleaved into: Gliomedin shedded ectodomain]

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q6ZMI3

  • Expression Region

    1-551aa

  • AA Sequence

    MARGAEGGRGDAGWGLRGALAAVALLSALNAAGTVFALCQWRGLSSALRALEAQRGREQREDSALRSFLAELSRAPRGASAPPQDPASSARNKRSHSGEPAPHIRAESHDMLMMMTYSMVPIRVMVDLCNSTKGICLTGPSGPPGPPGAGGLPGHNGLDGQPGPQGPKGEKGANGKRGKMGIPGAAGNPGERGEKGDHGELGLQGNEGPPGQKGEKGDKGDVSNDVLLAGAKGDQGPPGPPGPPGPPGPPGPPGSRRAKGPRQPSMFNGQCPGETCAIPNDDTLVGKADEKASEHHSPQAESMITSIGNPVQVLKVTETFGTWIRESANKSDDRIWVTEHFSGIMVKEFKDQPSLLNGSYTFIHLPYYFHGCGHVVYNNSLYYHKGGSNTLVRFEFGQETSQTLKLENALYFDRKYLFANSKTYFNLAVDEKGLWIIYASSVDGSSILVAQLDERTFSVVQHVNTTYPKSKAGNAFIARGILYVTDTKDMRVTFAFDLLGGKQINANFDLRTSQSVLAMLAYNMRDQHLYSWEDGHLMLYPVQFLSTTLNQ

  • Molecular Weight

    58.9 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

Quality inspection process

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Protein Description

GLDN (Gliomedin) is a prominent protein highly involved in the formation of peripheral nervous system (PNS) nodes of Ranvier, which are critical for saltatory conduction and overall myelination of axons. Its role extends to the interaction with other cellular components during the assembly of the nodal structure, highlighting its importance in maintaining neural function and integrity. Research into GLDN and its recombinant forms has gained momentum due to its implications in neuromuscular diseases and disorders characterized by demyelination, such as multiple sclerosis and Charcot-Marie-Tooth disease. The development of recombinant GLDN proteins enables detailed studies of its structural and functional properties, facilitating better understanding of its mechanism of action in the physiological context. Additionally, these recombinant proteins can serve as valuable tools for exploring therapeutic strategies aimed at promoting remyelination and recovery in affected neurological diseases. Moreover, insights from GLDN studies may contribute to bioengineering applications in neural tissue repair and regenerative medicine, ultimately paving the way for innovative treatment options for various neurodegenerative disorders.

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