Analytical Data
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Gene name
PFKP
- Application
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Alternative Names
PFKP；PFKF；ATP-dependent 6-phosphofructokinase. platelet type
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q01813
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Expression Region
1-784aa
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AA Sequence
MDADDSRAPKGSLRKFLEHLSGAGKAIGVLTSGGDAQGMNAAVRAVVRMG IYVGAKVYFIYEGYQGMVDGGSNIAEADWESVSSILQVGGTIIGSARCQA FRTREGRLKAACNLLQRGITNLCVIGGDGSLTGANLFRKEWSGLLEELAR NGQIDKEAVQKYAYLNVVGMVGSIDNDFCGTDMTIGTDSALHRIIEVVDA IMTTAQSHQRTFVLEVMGRHCGYLALVSALACGADWVFLPESPPEEGWEE QMCVKLSENRARKKRLNIIIVAEGAIDTQNKPITSEKIKELVVTQLGYDT RVTILGHVQRGGTPSAFDRILASRMGVEAVIALLEATPDTPACVVSLNGN HAVRLPLMECVQMTQDVQKAMDERRFQDAVRLRGRSFAGNLNTYKRLAIK LPDDQIPKTNCNVAVINVGAPAAGMNAAVRSAVRVGIADGHRMLAIYDGF DGFAKGQIKEIGWTDVGGWTGQGGSILGTKRVLPGKYLEEIATQMRTHSI NALLIIGGFEAYLGLLELSAAREKHEEFCVPMVMVPATVSNNVPGSDFSI GADTALNTITDTCDRIKQSASGTKRRVFIIETMGGYCGYLANMGGLAAGA DAAYIFEEPFDIRDLQSNVEHLTEKMKTTIQRGLVLRNESCSENYTTDFI YQLYSEEGKGVFDCRKNVLGHMQQGGAPSPFDRNFGTKISARAMEWITAK LKEARGRGKKFTTDDSICVLGISKRNVIFQPVAELKKQTDFEHRIPKEQW WLKLRPLMKILAKYKASYDVSDSGQLEHVQPWSV
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Molecular Weight
112 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PFKP (phosphofructokinase platelet) is a key enzyme involved in glycolysis, catalyzing the phosphorylation of fructose-6-phosphate to produce fructose-1,6-bisphosphate, which is crucial for energy production in cells. Research into PFKP has gained attention due to its regulatory role in cellular metabolism and its potential implications in various diseases, including cancer, where altered glycolytic pathways are common. The study of recombinant PFKP offers insights into its structure-function relationships, enzymatic mechanisms, and the impact of mutations that may influence its activity. Understanding these aspects is essential for developing therapeutic strategies that target metabolic dysregulation in diseases. Furthermore, recombinant PFKP can serve as a valuable tool in biotechnological applications, including metabolic engineering and synthetic biology. Investigating the enzymatic properties of PFKP through reconstitution and mutagenesis allows researchers to delineate the factors that govern its activity and regulation, potentially leading to novel interventions in metabolic disorders. Overall, the exploration of PFKP and its recombinant forms provides a rich avenue for advancing our knowledge of metabolic physiology and its clinical ramifications.
