Analytical Data
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基因名
ADAMTS4
- Application
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别名
ADAMTS4；KIAA0688；A disintegrin and metalloProteinase with thrombospondin motifs 4
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O75173
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表达区间
1-339aa
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氨基酸序列
MSQTGSHPGRGLAGRWLWGAQPCLLLPIVPLSWLVWLLLLLLASLLPSAR LASPLPREEEIVFPEKLNGSVLPGSGAPARLLCRLQAFGETLLLELEQDS GVQVEGLTVQYLGQAPELLGGAEPGTYLTGTINGDPESVASLHWDGGALL GVLQYRGAELHLQPLEGGTPNSAGGPGAHILRRKSPASGQGPMCNVKAPL GSPSPRPRRAKRFASLSRFVETLVVADDKMAAFHGAGLKRYLLTVMAAAA KAFKHPSIRNPVSLVVTRLVILGSGEEGPQVGPSAAQTLRSFCAWQRGLN TPEDSDPDHFDTAILFTRQVRPQSAPQAMHCTILRSATT
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分子量
62 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ADAMTS4, a member of the disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family, is crucial for extracellular matrix (ECM) remodeling, particularly in the context of cartilage degradation. Its enzymatic activity predominantly targets aggrecan, a key component of the cartilage matrix, leading to its proteolytic cleavage. This process is significantly implicated in the development and progression of osteoarthritis (OA) and other degenerative joint diseases, where the balance between aggrecan synthesis and degradation is disrupted. Research has shown that ADAMTS4 expression is upregulated in the cartilage of OA patients, highlighting its role in disease pathology. The investigation of recombinant ADAMTS4 protein has gained attention as it offers insights into the mechanisms of cartilage breakdown and potential therapeutic strategies. By studying its structure, function, and interactions with other ECM components, researchers aim to uncover novel targets for intervention, potentially leading to the development of inhibitors that could slow down or prevent cartilage degradation in OA. Thus, the study of recombinant ADAMTS4 not only enhances our understanding of cartilage biology but also aids in addressing the clinical challenges posed by arthritic conditions.
