Analytical Data
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Gene name
NSMASE
- Application
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Alternative Names
NSMASE；Sphingomyelin phosphodiesterase 3
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60906
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Expression Region
1-423aa
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AA Sequence
MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEV WSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTL NGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLA HRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDA YLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYI SCKSFETTTGFDPHSGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERS PLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGA GEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQ DLGPEPQPALLLGQQEGDRTKEQ
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Molecular Weight
73 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NSMASE, or Neuronal Smase, is an important enzyme that plays a crucial role in the metabolism of sphingolipids, which are vital components of cell membranes and are involved in various biological processes, including cell signaling, apoptosis, and inflammation. The study of NSMASE is particularly significant due to its implications in neurobiology and potential associations with neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. Recent research has indicated that alterations in sphingolipid metabolism can lead to disruptions in neuronal function and survival. Therefore, the recombinant expression and purification of NSMASE provide valuable insights into its structure-function relationship and its role in lipid metabolism. Investigating NSMASE through recombinant protein techniques facilitates the development of specific inhibitors that could serve as therapeutic agents, offering new avenues for treating diseases associated with sphingolipid dysregulation. Furthermore, understanding the regulatory mechanisms of NSMASE may reveal novel targets for drug development aimed at neuroprotection and the modulation of cellular responses to stress. Overall, the study of NSMASE and its recombinant protein forms is essential for unraveling the complexities of sphingolipid metabolism in the nervous system and for advancing potential therapies for related disorders.
