Analytical Data
-
基因名
MGAT5
- Application
-
别名
MGAT5；GGNT5；Alpha-1.6-mannosylglycoProtein 6-beta-N-acetylglucosaminyltransferase A
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q09328
-
表达区间
189-741aa
-
氨基酸序列
LSEVENWCPH LPWRAKNPYE EADHNSLAEI RTDFNILYSM MKKHEEFRWM RLRIRRMADA WIQAIKSLAE KQNLEKRKRK KVLVHLGLLT KESGFKIAET AFSGGPLGEL VQWSDLITSL YLLGHDIRIS ASLAELKEIM KKVVGNRSGC PTVGDRIVEL IYIDIVGLAQ FKKTLGPSWV HYQCMLRVLD SFGTEPEFNH ANYAQSKGHK TPWGKWNLNP QQFYTMFPHT PDNSFLGFVV EQHLNSSDIH HINEIKRQNQ SLVYGKVDSF WKNKKIYLDI IHTYMEVHAT VYGSSTKNIP SYVKNHGILS GRDLQFLLRE TKLFVGLGFP YEGPAPLEAI ANGCAFLNPK FNPPKSSKNT DFFIGKPTLR ELTSQHPYAE VFIGRPHVWT VDLNNQEEVE DAVKAILNQK IEPYMPYEFT CEGMLQRINA FIEKQDFCHG QVMWPPLSAL QVKLAEPGQS CKQVCQESQL ICEPSFFQHL NKDKDMLKYK VTCQSSELAK DILVPSFDPK NKHCVFQGDL LLFSCAGAHP RHQRVCPCRD FIKGQVALCK DCL
-
分子量
65 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
MGAT5, or Mannosyltransferase 5, is a pivotal enzyme involved in the synthesis of complex N-glycans, which play crucial roles in glycoprotein function, stability, and recognition processes. Its enzymatic activity is essential for the proper folding of glycoproteins and influences various biological mechanisms, including cell adhesion, immune response, and tumor progression. In recent years, research has increasingly focused on MGAT5 due to its involvement in oncogenesis and metastasis, particularly in certain cancers such as prostate and breast cancer. The overexpression of MGAT5 has been correlated with enhanced tumor aggressiveness and poor patient prognosis, making it a potential biomarker and therapeutic target. By studying MGAT5 recombinant proteins, researchers aim to elucidate its mechanistic roles in glycosylation pathways and its impact on cellular behaviors. Understanding the structure and function of MGAT5 can provide insights into the development of glycan-based therapeutic strategies. Moreover, recombinant MGAT5 proteins can serve as valuable tools for exploring glycan function in various biological contexts, thereby contributing to advancements in glycobiology and therapeutic interventions for glycan-associated diseases.
