Analytical Data
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Gene name
RPIP8
- Application
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Alternative Names
RUNDC3A; RAP2IP; RPIP8; RUN domain-containing protein 3A; Rap2-interacting protein 8; RPIP-8
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q59EK9
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Expression Region
1-446 aa
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AA Sequence
MEASFVQTTM ALGLSSKKAS SRNVAVERKN LITVCRFSVK TLLEKYTAEP IDDSSEEFVN FAAILEQILS HRFKACAPAG PVSWFSSDGQ RGFWDYIRLA CSKVPNNCVS SIENMENIST ARAKGRAWIR VALMEKRMSE YITTALRDTR TTRRFYDSGA IMLRDEATIL TGMLIGLSAI DFSFCLKGEV LDGKTPVVID YTPYLKFTQS YDYLTDEEER HSAESSTSED NSPEHPYLPL VTDEDSWYSK WHKMEQKFRI VYAQKGYLEE LVRLRESQLK DLEAENRRLQ LQLEEAAAQN QREKRELEGV ILELQEQLTG LIPSDHAPLA QGSKELTTPL VNQWPSLGTL NGAEGASNSK LYRRHSFMST EPLSAEASLS SDSQRLGEGT RDEEPWGPIG KDPTPSMLGL CGSLASIPSC KSLASFKSNE CLVSDSPEGS PALSPS
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Molecular Weight
49.7 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RPIP8 (RNA phosphatase interacting protein 8) is a protein implicated in various cellular processes, particularly in the regulation of RNA metabolism and signal transduction pathways. Recent studies have shown that RPIP8 plays a critical role in the modulation of RNA stability and translation, thus influencing gene expression and cellular responses to environmental stresses. Understanding the function of RPIP8 is crucial as it may be linked to several pathological conditions, including cancer and neurodegenerative diseases, where dysregulation of RNA processing is often observed. Research on RPIP8 often involves the development of recombinant proteins to study its biochemical properties and interaction with other molecules in vitro. This includes the use of techniques such as recombinant DNA technology, bacterial expression systems, and purification methods, allowing for the characterization of RPIP8’s enzymatic activities and its role in cellular signaling networks. Furthermore, analyzing the structural features of RPIP8 through techniques like X-ray crystallography or nuclear magnetic resonance (NMR) provides insights into its functional mechanisms. Given its significance in RNA regulation, continued research on RPIP8 not only enhances our understanding of basic cellular biology but also holds potential for therapeutic applications targeting relevant diseases.
