Analytical Data
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基因名
ldh1
- Application
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别名
ldh1；Laforin
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种属
E.coli
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P38139
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表达区间
1-375aa
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氨基酸序列
MNMAERAEATKSWSCEPLSGKTLEEIVQNAENAADLVAYIRKPEVDLDFRLKFIAEHEEFFNVQLSDRNSRIRTCHNLSDKGIRGDTVFVFVPGLAGNLEQFEPLLELVDSDQKAFLTLDLPGFGHSSEWSDYPMLKVVELIFVLVCDVLRKWSTAVPNNDNVNPFNGHKIVLVGHSMGCFLACHLYEQHMADTKAVQTLVLLTPPKAHIEQLSKDKHIIQWALYGVFKLPWLFDVYRNKFDQVKGLQSSGIKQYFYQQGDDVKLKYRKFWQFKNNISNKSRTIIGYLLGWETVDWVKFNGVLTQTDMKQKIIIFGAEKDPIAPIENLEFYKQTINKECLRKVIILPDCSHNLCFDRPELVCENFQREVIDNSKL
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分子量
43.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Lactate dehydrogenase (LDH) is an important enzyme involved in the metabolic process of converting lactate to pyruvate and vice versa, playing a critical role in anaerobic respiration and energy production. Among its five isoforms, LDH1 is predominantly expressed in the heart and red blood cells, making it a key player in cardiac metabolism and a potential biomarker for myocardial injury. Due to its significance in clinical diagnostics, researchers have focused on the recombinant production of LDH1 in various expression systems. The recombinant LDH1 protein allows for detailed studies of its enzymatic properties, structural characteristics, and interactions with other biomolecules. This research is pivotal in understanding the enzyme’s role in physiological and pathological conditions, including ischemic heart disease and cancer metabolism, as altered LDH activity is often associated with tumor progression and poor prognosis. Furthermore, the availability of purified recombinant LDH1 facilitates the development of therapeutic strategies and the exploration of its potential as a drug target. Understanding the mechanisms that regulate LDH1 activity could lead to innovative approaches in treating diseases associated with metabolic dysregulation. Thus, the study of recombinant LDH1 protein not only enhances our fundamental knowledge of metabolic pathways but also underscores its clinical relevance in biomarker development and therapeutic intervention.
