Analytical Data
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Gene name
ctfA
- Application
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Alternative Names
ctfA；Acetoacetyl-CoA:acetate/butyrate CoA transferase alpha subunit
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P33752
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Expression Region
1-218aa
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AA Sequence
MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIKNLTIISNDTCYPNTGIGKLISNNQVKKLIASYIGSNPDTGKKLFNNELEVELSPQGTLVERIRAGGSGLGGVLTKTGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVDEAGNTFYKGTTKNFNPYMAMAAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA
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Molecular Weight
23.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CtfA protein, encoded by the ctfA gene, plays a crucial role in various biological processes, particularly in bacterial cell division and biofilm formation. Research into CtfA primarily centers around its function as an essential protein in the assembly of the divisome, the complex responsible for bacterial cytokinesis. Deletion or mutation of the ctfA gene often leads to significant defects in cell morphology and division, highlighting its importance in maintaining cellular integrity and normal growth patterns. Beyond its role in cell division, CtfA has also been implicated in the regulation of biofilm formation, a critical factor in bacterial survival and persistence in various environments. Studies have indicated that CtfA interacts with other proteins within the divisome and potentially influences the dynamics of this assembly, thereby impacting the overall process of cell division. The investigation into CtfA not only enhances our understanding of bacterial physiology but also holds potential implications for the development of novel antimicrobial strategies aimed at disrupting bacterial growth and biofilm development. Understanding the structural and functional properties of CtfA could lead to innovative approaches in combating bacterial infections and biofilm-related complications, making it a significant target for future research in microbiology and biotechnology.
