Recombinant Rhesus Macaque PDGF R beta Protein(HEK293), C-His

PDGF R beta

PDGF R beta, a tyrosine-protein kinase receptor, crucially regulates embryonic development, overseeing cell processes like proliferation, survival, differentiation, chemotaxis, and migration. Especially vital in blood vessel development, it facilitates pericyte and smooth muscle cell proliferation, migration, and recruitment to endothelial cells, playing a significant role in blood vessel formation and maintenance. PDGF R beta Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived PDGF R beta protein, expressed by HEK293 , with C-His labeled tag.

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Measured by its ability to inhibit the biological activity of PDGF-BB using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 2.236 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF-BB, corresponding to a specific activity is 447.22 Unit/mg. Measured by its ability to inhibit the biological activity of PDGF-BB using NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 2.236 µg/mL in the presence of 50 ng/mL Recombinant Human PDGF-BB, corresponding to a specific activity is 447.22 unit/mg.

Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-1; CD140b; PDGFRB

Rhesus Macaque

HEK293

C-His

Greater than 95% as determined by reducing SDS-PAGE.

F7BTH1

L33-F530

LVITPPGPELILNVSSTFVLTCSGSAPVVWERMSQELPQEMAKAQDNTFSSVLTLTNLTGLDTGEYFCTYNDSRGLEPDERKRLYIFVPDPTMGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDIALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWMYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGALQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPMLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDMQEVIVVPHSLPF

Partial

80-96 kDa.

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.