Analytical Data
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Gene name
atoD
- Application
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Alternative Names
atoD；COL29A1；VWA4；Collagen alpha-5(VI) chain
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Species
E.coli
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P76458
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Expression Region
1-220aa
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AA Sequence
MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGVRDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGTLIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCDTLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQESK
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Molecular Weight
30.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of atoD recombinant proteins has emerged as a significant area of interest within microbiology and biotechnology due to their crucial role in bacterial stress responses and pathogenicity. atoD is part of the ato system, which is involved in the metabolism of short-chain fatty acids, particularly in the hydrolysis of acyl-CoA compounds. This system enables bacteria to utilize alternative carbon sources, making them more versatile in various environments, including those with limited nutrients. The Ato system's complex regulatory mechanisms highlight its importance in bacterial adaptation and survival, which is particularly pertinent in pathogenic strains that must evade host defenses. The production and characterization of atoD recombinant proteins provide insights into their structure-function relationships and enzymatic activities. This research not only enhances our understanding of bacterial metabolism and stress resilience but also has potential implications for developing novel antimicrobial strategies. By elucidating the molecular characteristics and interactions of atoD, scientists aim to identify new targets for drug development and to engineer bacteria with improved metabolic capabilities for bioremediation and industrial applications. Overall, the exploration of atoD recombinant proteins represents a multidisciplinary approach combining microbiology, biochemistry, and genetic engineering, fostering advancements in both fundamental science and applied technologies.
