Analytical Data
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基因名
ACC1
- Application
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别名
ACC1；Activating signal cointegrator 1 complex subunit 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q00955
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表达区间
217-775aa
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氨基酸序列
TGLVSVDDDIYQKGCCTSPEDGLQKAKRIGFPVMIKASEGGGGKGIRQVEREEDFIALYHQAANEIPGSPIFIMKLAGRARHLEVQLLADQYGTNISLFGRDCSVQRRHQKIIEEAPVTIAKAETFHEMEKAAVRLGKLVGYVSAGTVEYLYSHDDGKFYFLELNPRLQVEHPTTEMVSGVNLPAAQLQIAMGIPMHRISDIRTLYGMNPHSASEIDFEFKTQDATKKQRRPIPKGHCTACRITSEDPNDGFKPSGGTLHELNFRSSSNVWGYFSVGNNGNIHSFSDSQFGHIFAFGENRQASRKHMVVALKELSIRGDFRTTVEYLIKLLETEDFEDNTITTGWLDDLITHKMTAEKPDPTLAVICGAATKAFLASEEARHKYIESLQKGQVLSKDLLQTMFPVDFIHEGKRYKFTVAKSGNDRYTLFINGSKCDIILRQLSDGGLLIAIGGKSHTIYWKEEVAATRLSVDSMTTLLEVENDPTQLRTPSPGKLVKFLVENGEHIIKGQPYAEIEVMKMQMPLVSQENGIVQLLKQPGSTIVAGDIMAIMTLDDPSKV
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分子量
67.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACC1 (acetyl-CoA carboxylase 1) is a pivotal enzyme involved in fatty acid synthesis and regulation of lipid metabolism, making it a significant target for understanding metabolic diseases such as obesity and type 2 diabetes. The enzyme catalyzes the conversion of acetyl-CoA to malonyl-CoA, the first committed step in the fatty acid biosynthesis pathway. Aberrant ACC1 activity is associated with various metabolic disorders, leading to increased lipogenesis and altered energy homeostasis. Recent studies have focused on the structural and functional characterization of ACC1, including its regulatory mechanisms and the effects of post-translational modifications. Understanding the intricate regulation of ACC1 offers potential therapeutic avenues for metabolic diseases, as inhibitors of ACC1 could provide an innovative strategy for managing obesity and associated disorders. Moreover, recombinant expression of ACC1 in bacterial or eukaryotic systems has facilitated the purification and characterization of this enzyme, paving the way for high-throughput screening of small-molecule inhibitors. The ongoing research into ACC1 not only sheds light on its biological significance but also underscores its potential as a drug target in the treatment of metabolic syndromes, thereby contributing to the burgeoning field of metabolic engineering and therapeutic development.
