Analytical Data
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基因名
TDO2
- Application
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别名
TDO2；TDO；Tryptophan 2.3-dioxygenase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P48775
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表达区间
1-406aa
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氨基酸序列
MSGCPFLGNNFGYTFKKLPVEGSEEDKSQTGVNRASKGGLIYGNYLHLEK VLNAQELQSETKGNKIHDEHLFIITHQAYELWFKQILWELDSVREIFQNG HVRDERNMLKVVSRMHRVSVILKLLVQQFSILETMTALDFNDFREYLSPA SGFQSLQFRLLENKIGVLQNMRVPYNRRHYRDNFKGEENELLLKSEQEKT LLELVEAWLERTPGLEPHGFNFWGKLEKNITRGLEEEFIRIQAKEESEEK EEQVAEFQKQKEVLLSLFDEKRHEHLLSKGERRLSYRALQGALMIYFYRE EPRFQVPFQLLTSLMDIDSLMTKWRYNHVCMVHRMLGSKAGTGGSSGYHY LRSTVSDRYKVFVDLFNLSTYLIPRHWIPKMNPTIHKFLYTAEYCDSSYF SSDESD
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分子量
48 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TDO2, or tryptophan 2,3-dioxygenase, is an enzyme that plays a crucial role in the catabolism of tryptophan, an essential amino acid. It is primarily found in the liver and is responsible for the oxidative cleavage of tryptophan, which leads to the production of kynurenine and other metabolites involved in various biological processes. Research on TDO2 has gained significant attention due to its implications in several physiological and pathological conditions, including neurodegenerative diseases, cancer, and immunological disorders. Elevated levels of TDO2 have been associated with immune suppression and tumor progression, as the kynurenine pathway can modulate immune responses and promote a tolerogenic environment favorable to tumor growth. Moreover, alterations in tryptophan metabolism are linked to mood disorders and cognitive function, suggesting a potential role for TDO2 in psychiatric conditions. Thus, the study of TDO2 recombinant proteins aims to elucidate the enzyme's structure, function, and biological significance, paving the way for potential therapeutic interventions targeting the kynurenine pathway. Understanding TDO2's mechanisms may lead to novel strategies for treating diseases where tryptophan metabolism is disrupted, offering prospects for improved patient outcomes and novel pharmacological approaches. The ongoing research may also help identify biomarkers for disease prognosis and response to treatment, contributing to personalized medicine in the management of complex health issues.
