Analytical Data
-
Gene name
PRR5-ARHGAP8
- Application
-
Alternative Names
PRR5-ARHGAP8 readthrough
-
Species
Human
-
Source
E. coli
-
Tag
GST-tag at N-terminal
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
B1AHC3
-
Expression Region
1-469 aa
-
AA Sequence
MVILRDKIRFYEELQRDKAAAAAVLGAVRKRPSVVPMAGQDPALSTSHPFYDVARHGILQVAGDDRFGRRVVTFSCCRMPPSHELDHQRLLEYLKYTLDQYVENDYTIVYFHYGLNSRNKPSLGWLQSAYKEFDRKYKKNLKALYVVHPTSFIKVLWNILKPLISHKFGKKVIYFNYLSELHEHLKYDQLVIPPEVLRYDEKLQSLHEGRTPPPTKTPPPRPPLPTQQFGVSLQYLKDKNQGELIPPVLRFTVTYLREKGLRTEGLFRRSASVQTVREIQRLYNQGKPVNFDDYGDIHIPAVILKTFLRELPQPLLTFQAYEQILGITCVESSLRVTGCRQILRSLPEHNYVVLRYLMGFLHAVSRESIFNKMNSSNLACVFGLNLIWPSQGVSSLSALVPLNMFTELLIEYYEKIFSTPEAPGEHGLAPWEQGSRAAPLQEAVPRTQATGLTKPTLPPSPLMAARRRL
-
Molecular Weight
51.6 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRR5-ARHGAP8 is a fusion protein that has garnered significant interest in the field of molecular biology and cancer research due to its potential role in cell signaling and tumorigenesis. PRR5 (Proline-rich protein 5) is known to be involved in the regulation of the mTOR (mechanistic target of rapamycin) signaling pathway, which is crucial for cell growth and metabolism. ARHGAP8 (Rho GTPase-activating protein 8), on the other hand, plays a vital role in regulating the Rho family of GTPases, influencing cell migration, adhesion, and cytoskeletal dynamics. The combination of these two proteins into a single fusion protein may lead to the dysregulation of both pathways, contributing to cancer progression. Studies have identified that alterations in either PRR5 or ARHGAP8 can affect cellular processes associated with malignancy. Additionally, the research aims to elucidate the molecular mechanisms underlying the functional properties of PRR5-ARHGAP8, especially its impact on cancer cell behavior and response to therapies. Understanding this fusion protein could provide insights into novel therapeutic approaches for targeting cancerous cells, thus highlighting its importance in both basic research and potential clinical applications. Investigating the structure-function relationships and signaling pathways associated with PRR5-ARHGAP8 could reveal new biomarkers for cancer diagnosis and prognosis, paving the way for innovative strategies to combat this complex disease.
