Analytical Data
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基因名
PNO1
- Application
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别名
Partner of NOB1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NRX1
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表达区间
1-252 aa
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氨基酸序列
MESEMETQSARAEEGFTQVTRKGGRRAKKRQAEQLSAAGEGGDAGRMDTEEARPAKRPVFPPLCGDGLLSGKEETRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNVEIRTCKETKDVSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEITDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVHILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSADRF
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分子量
34.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PNO1 (Promoter of Nonsense Mediated mRNA Decay 1) is a crucial protein involved in the regulation of mRNA decay processes, specifically the nonsense-mediated decay (NMD) mechanism. NMD is a quality control pathway that degrades mRNAs containing premature stop codons, preventing the synthesis of truncated proteins that might be detrimental to cellular function. The study of PNO1 has gained significance due to its potential implications in various diseases, including cancer and genetic disorders wherein faulty mRNA processing contributes to pathogenesis. Recent research has highlighted PNO1's role in enhancing the efficiency of NMD, thereby suggesting that it may act as a regulatory factor, influencing cellular responses to stress and potentially impacting gene expression regulation. Understanding the structural and functional characteristics of PNO1 can lead to insights into its molecular interactions and mechanisms of action, which could open up new avenues for therapeutic interventions targeting NMD-related pathways. As researchers continue to investigate the multifaceted roles of PNO1, the implications for both fundamental biology and clinical applications remain an active area of exploration, potentially leading to novel strategies for managing diseases associated with mRNA processing defects.
