Analytical Data
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Gene name
PMS1
- Application
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Alternative Names
DNA mismatch repair protein PMS1; FLJ98259; HNPCC3; hPMS1; Human homolog of yeast mutL; Mismatch repair gene PMSL1; pms1; PMS1 postmeiotic segregation increased 1 (S. cerevisiae); PMS1 postmeiotic segregation increased 1; PMS1 protein homolog 1; PMS1_HUMAN; PMSL1; Rhabdomyosarcoma antigen MU RMS 40.10B; Rhabdomyosarcoma antigen MU RMS 40.10E
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P54277
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Expression Region
1-166 aa
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AA Sequence
MKQLPAATVRLLSSSQIITSVVSVVKELIENSLDAGATSVDVKLENYGFDKIEVRDNGEGIKAVDAPVMAMKYYTSKINSHEDLENLTTYGFRGEALGSICCIAEVLITTRTAADNFSTQYVLDGSGHILSQKPSHLGQGKKVALYTNILYLFCLNCWFKKKKVTR
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Molecular Weight
44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PMS1, or PMS1 homolog 1, is a gene that plays a critical role in DNA mismatch repair (MMR), a fundamental cellular process that ensures the fidelity of DNA replication. Deficiencies in MMR proteins, like PMS1, can lead to increased mutation rates and are associated with various cancers, particularly hereditary non-polyposis colorectal cancer (HNPCC), also known as Lynch syndrome. The study of PMS1 recombinant proteins is crucial for understanding the molecular mechanisms underlying MMR and its role in maintaining genomic stability. By investigating the structure and function of PMS1, researchers aim to elucidate its interactions with other MMR components and identify the consequences of its dysfunction. Recombinant PMS1 proteins can be produced in a laboratory setting to facilitate in-depth biochemical assays and structural analyses, paving the way for insights into how mutations in PMS1 contribute to tumorigenesis. Furthermore, this research holds potential therapeutic implications, as identifying small molecules or compounds that can restore or enhance the function of defective MMR proteins could lead to novel strategies for cancer treatment and prevention. Overall, the exploration of PMS1 recombinant proteins represents a vital area of research that bridges fundamental biology and clinical applications in oncology.
