Analytical Data
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基因名
PFKFB3
- Application
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别名
6 phosphofructo 2 kinase/ fructose 2.6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2.6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2.6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2.6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate.2 kinase/fructose 2; 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2.6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q16875
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表达区间
1-520 aa
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氨基酸序列
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLAKEGGQ IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR ISCYEASYQP LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY LCRHGENEHN LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVC THRERSEDAK KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT QLPGQNMKGS RSSADSSRKH
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分子量
59.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PFKFB3, or 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3, is a critical enzyme that plays a pivotal role in the regulation of glucose metabolism, particularly in controlling the levels of fructose 2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1 (PFK-1). This enzyme is central to the glycolytic pathway, influencing cancer cell metabolism and survival, particularly in the context of the Warburg effect, where cancer cells preferentially utilize glycolysis for energy production even in the presence of oxygen. Recent studies have highlighted the significance of PFKFB3 in various pathological conditions, including type 2 diabetes, metabolic syndrome, and different types of cancers, making it a promising target for therapeutic intervention. The recombinant expression of PFKFB3 facilitates the study of its enzymatic activity and regulatory mechanisms, providing insights into its function and potential as a biomarker for disease progression. Furthermore, developing specific inhibitors or modulators of PFKFB3 could lead to novel strategies for targeting metabolic dysregulation in cancer and other diseases. Understanding the structure and function of PFKFB3 through recombinant protein studies is therefore essential for elucidating its role in cellular metabolism and its potential as a therapeutic target.
