Analytical Data
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Gene name
EIF4B
- Application
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Alternative Names
EIF4B；Eukaryotic translation initiation factor 4B
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P23588
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Expression Region
1-611aa
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AA Sequence
MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGD VSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFL GNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSL LSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWR ARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPCRDMD RYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYED RYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSAS TSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRP RERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENET LNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDT EQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDG GNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDG EDENEGEDYAE
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Molecular Weight
93 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
EIF4B, or Eukaryotic Translation Initiation Factor 4B, is a critical protein involved in the initiation of eukaryotic translation, playing a pivotal role in the recruitment of ribosomes to mRNA. This protein is essential for the formation of the translation initiation complex by binding to the mRNA cap structure and interacting with other components of the translation machinery. Given its crucial function, abnormalities in EIF4B expression or activity can have significant implications for cellular processes, including growth, differentiation, and response to stress. Research on EIF4B has gained momentum due to its potential connections to various diseases, including cancer, where upregulation of translation factors like EIF4B is often observed, leading to uncontrolled cell proliferation. Furthermore, EIF4B’s involvement in the regulation of mRNA translation during viral infections evokes interest, as many viruses hijack the host's translational machinery to facilitate their replication. This has prompted studies aimed at understanding the molecular mechanisms governing EIF4B's function, its interaction with other translational components, and its regulation under different cellular conditions. The characterization of EIF4B and its signaling pathways not only enhances our understanding of fundamental biological processes but also opens avenues for the development of novel therapeutic strategies targeting aberrant translation associated with diseases. Hence, the study of EIF4B recombinant proteins holds promise for elucidating the intricacies of translation regulation and its implications in health and disease.
