Analytical Data
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基因名
ACSL1
- Application
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别名
ACSL1;FACL1;FACL2;LACS;Long-chain-fatty-acid--CoA ligase 1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P33121
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表达区间
48-145aa
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氨基酸序列
PKPLKPPCDLSMQSVEVAGSGGARRSALLDSDEPLVYFYDDVTTLYEGFQ RGIQVSNNGPCLGSRKPDQPYEWLSYKQVAELSECIGSALIQKGFKTA
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分子量
36 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACSL1, or acyl-CoA synthetase long-chain family member 1, plays a critical role in fatty acid metabolism and is involved in the activation of long-chain fatty acids to form acyl-CoA derivatives. Its significance extends beyond metabolism, as it has been implicated in various physiological processes, including membrane lipid synthesis, energy homeostasis, and signaling pathways. Dysregulation of ACSL1 has been associated with metabolic disorders, cancer, and cardiovascular diseases, making it a target of considerable interest in biomedical research. The recombinant expression of ACSL1 allows for the detailed study of its enzymatic activity and regulatory mechanisms, facilitating the understanding of its role in lipid metabolism and its contributions to disease pathogenesis. By producing recombinant ACSL1, researchers can investigate the protein's structure-function relationships, determine its specific substrate preferences, and explore its interactions with other metabolic pathways. This knowledge is vital for developing therapeutic strategies aimed at modulating ACSL1 activity in disease contexts, thereby providing insights into potential interventions for metabolic syndrome, obesity, and related disorders. Additionally, studies on ACSL1 can contribute to the broader understanding of lipid homeostasis and its implications in health and disease.












