Analytical Data
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Gene name
SKA1
- Application
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Alternative Names
SKA1;C18orf24;Spindle and kinetochore-associated Protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96BD8
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Expression Region
1-255aa
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AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMASSDLE QLCSHVNEKI GNIKKTLSLR NCGQEPTLKT VLNKIGDEII VINELLNKLE LEIQYQEQTN NSLKELCESL EEDYKDIEHL KENVPSHLPQ VTVTQSCVKG SDLDPEEPIK VEEPEPVKKP PKEQRSIKEM PFITCDEFNG VPSYMKSRLTYNQINDVIKE INKAVISKYK ILHQPKKSMN SVTRNLYHRF IDEETKDTKG RYFIVEADIK EFTTLKADKK FHVLLNILRH CRRLSEVRGGGLTRYVIT
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Molecular Weight
32 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of SKA1 (Spindle and Kinetochore Associated 1) recombinant protein has gained significant attention due to its crucial role in the proper functioning of the mitotic spindle during cell division. SKA1 is a component of the kinetochore, a protein structure on chromatids where the spindle fibers attach during mitosis. Research has revealed that SKA1 is vital for ensuring accurate chromosome segregation, preventing aneuploidy, and maintaining genomic stability, which is particularly important in cancer biology. Abnormal expression or function of SKA1 has been associated with various cancers, making it a potential target for therapeutic intervention. This recombinant protein is often synthesized in host systems such as bacteria or yeast for further analysis, enabling the study of its structure, interactions, and functional properties. Investigating SKA1 through recombinant technology not only deepens our understanding of its biological roles but also opens avenues for the development of novel cancer treatments that could exploit its essential functions in mitosis. Consequently, the production and characterization of SKA1 recombinant protein is a promising area of research with implications for both fundamental biology and clinical applications.
