Analytical Data
-
基因名
SKA1
- Application
-
别名
SKA1;C18orf24;Spindle and kinetochore-associated Protein 1
-
种属
Human
-
表达系统
E. coli
-
标签
His tag N-Terminus
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q96BD8
-
表达区间
1-255aa
-
氨基酸序列
MGSSHHHHHH SSGLVPRGSH MGSMASSDLE QLCSHVNEKI GNIKKTLSLR NCGQEPTLKT VLNKIGDEII VINELLNKLE LEIQYQEQTN NSLKELCESL EEDYKDIEHL KENVPSHLPQ VTVTQSCVKG SDLDPEEPIK VEEPEPVKKP PKEQRSIKEM PFITCDEFNG VPSYMKSRLTYNQINDVIKE INKAVISKYK ILHQPKKSMN SVTRNLYHRF IDEETKDTKG RYFIVEADIK EFTTLKADKK FHVLLNILRH CRRLSEVRGGGLTRYVIT
-
分子量
32 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of SKA1 (Spindle and Kinetochore Associated 1) recombinant protein has gained significant attention due to its crucial role in the proper functioning of the mitotic spindle during cell division. SKA1 is a component of the kinetochore, a protein structure on chromatids where the spindle fibers attach during mitosis. Research has revealed that SKA1 is vital for ensuring accurate chromosome segregation, preventing aneuploidy, and maintaining genomic stability, which is particularly important in cancer biology. Abnormal expression or function of SKA1 has been associated with various cancers, making it a potential target for therapeutic intervention. This recombinant protein is often synthesized in host systems such as bacteria or yeast for further analysis, enabling the study of its structure, interactions, and functional properties. Investigating SKA1 through recombinant technology not only deepens our understanding of its biological roles but also opens avenues for the development of novel cancer treatments that could exploit its essential functions in mitosis. Consequently, the production and characterization of SKA1 recombinant protein is a promising area of research with implications for both fundamental biology and clinical applications.
