Analytical Data
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Gene name
Proinsulin
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简介
Proinsulin is the precursor of insulin, a key hormone that regulates carbohydrate and lipid metabolism. Post-translational cleavage produces insulin, which consists of B and A chain peptides and a C peptide linked by disulfide bonds. Proinsulin Protein, Human (His) is the recombinant human-derived Proinsulin protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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Biological Activity
Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 0.5182μg/mL. Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is 0.5182 μg/mL. corresponding to a specific activity is 1929.7 units/mg.
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Alternative Names
IDDM2; ILPR; insulin; IRDN; MODY10; Proinsulin
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Species
Human
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
NP_000198.1
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Expression Region
F25-N110
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Protein Length
Full Length of Mature Protein
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Molecular Weight
10 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Proinsulin, the precursor of insulin, is a critical component in the regulation of glucose metabolism and is pivotal for the treatment of diabetes. The study of recombinant proinsulin has gained significance due to its potential for therapeutic applications. In the 1920s, insulin was first discovered as a treatment for diabetes, but its production was limited to animal sources until the advent of recombinant DNA technology in the 1970s. This breakthrough allowed for the production of human insulin and proinsulin using genetically modified organisms, such as bacteria and yeast, ensuring a consistent and safer source compared to animal-derived products. Research into proinsulin's structure and function has revealed its role in the formation of insulin, highlighting the importance of understanding this precursor for both basic science and clinical applications. Furthermore, proinsulin itself has generated interest for its potential use as a biomarker for early diabetes detection and as a therapeutic target. Current studies focus on enhancing methods for its production, purification, and characterization, which are essential for ongoing advancements in diabetes management. Investigating proinsulin has thus become a vital area of research, aimed at bridging the gap between basic endocrinology and the clinical treatment of diabetes, ultimately contributing to the development of novel therapies that improve patient outcomes.
