Analytical Data
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Gene name
NIPBL
- Application
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Alternative Names
CDLS; Colon tumor susceptibility 2; Delangin; DKFZp434L1319; FLJ11203; FLJ12597; FLJ13354; FLJ13648; FLJ44854; IDN 3; IDN 3 protein; IDN 3 protein isoform A; IDN 3 protein isoform B; IDN 3B; IDN3 B; IDN3 protein; IDN3 protein isoform A; IDN3 protein isoform B; IDN3B; Mis 4; Mis4; Nipbl; NIPBL_HUMAN; Nipped B homolog (Drosophila); Nipped B homolog; Nipped B like; Nipped B like protein; Nipped-B-like protein; Scc 2; SCC 2 homolog; Scc2; SCC2 homolog; Sister chromatid cohesion protein Mis4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6KC79
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Expression Region
66-175 aa
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AA Sequence
FHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT
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Molecular Weight
37.73 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NIPBL (Nipped-B homolog in Drosophila), a protein critical for chromatin remodeling and gene regulation, has garnered significant research interest due to its role in developmental processes and its association with Cornelia de Lange Syndrome (CdLS), a genetic disorder characterized by growth delay, intellectual disability, and distinctive facial features. NIPBL facilitates the assembly of cohesin complexes, which are essential for sister chromatid cohesion during cell division and also play a pivotal role in regulating gene expression by modulating chromatin architecture. The study of NIPBL is crucial for understanding the molecular mechanisms underlying CdLS, as mutations or deletions in the NIPBL gene are the most common cause of this syndrome. Recombinant NIPBL proteins are being expressed and purified to investigate their functional properties and interactions with other chromatin-associated proteins. By elucidating the structure and function of NIPBL, researchers aim to uncover the pathways disrupted in CdLS, providing insights into potential therapeutic targets. This research not only advances our understanding of chromatin biology and its implications in development but also holds promise for the development of interventions for CdLS and related disorders.
