Analytical Data
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Gene name
LR3 IGF-I/IGF-1
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简介
The LR3 IGF-I/IGF-1 protein is similar to insulin and has potent growth-promoting activity that exceeds the efficacy of insulin. As a potential physiological regulator, it stimulates glucose transport and glycogen synthesis in osteoblasts even at significantly lower concentrations than insulin. LR3 IGF-I/IGF-1 Protein, Human (83a.a, E51R) is the recombinant human-derived LR3 IGF-I/IGF-1 protein, expressed by E. coli , with tag free and E51R mutation.
- Application
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Biological Activity
Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 0.38-2.174 ng/mL. Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 0.3840 ng/mL, corresponding to a specific activity is 2.60×106 units/mg.
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Alternative Names
Long R3 IGF-I; Insulin-like growth factor I; MGF; Somatomedin-C; IBP1
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P05019-1 (G49-A118, E51R, with a 13 amino acid extension peptide
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Expression Region
MFPAMPLSSLFVN) at the N terminal
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Protein Length
Full Length of Isoform-1 Mature Protein
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Molecular Weight
9-11 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LR3 IGF-I, or Long R3 Insulin-like Growth Factor I, is a recombinant protein that serves as a potent analog of the naturally occurring IGF-I. This modification enhances its biological activity and prolongs its half-life in circulation. IGF-I plays a crucial role in growth and development, influencing cellular proliferation, differentiation, and survival. It is particularly important in muscle and bone development, making it a topic of interest in various fields, including endocrinology, oncology, and sports medicine. Research on LR3 IGF-I has shown promise in treating growth disorders, muscle wasting conditions, and metabolic diseases. Moreover, its potential application in anti-aging therapies and regenerative medicine has garnered significant attention. Unlike native IGF-I, which may have a short duration of action and is susceptible to rapid clearance, LR3 IGF-I provides sustained bioactivity through its structural modifications. This unique profile of LR3 IGF-I allows for more effective therapeutic interventions and underscores the need for continued investigation into its mechanisms of action, dosage optimization, and long-term effects in clinical settings. As researchers explore its applications, understanding the pharmacokinetics, safety profile, and biological effects of LR3 IGF-I remains pivotal for leveraging its full therapeutic potential.
