Analytical Data
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Gene name
IL-6RA
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简介
IL-6R alpha is a subunit alpha of IL-6 receptors, also shared by other interleukin receptors. IL-6R alpha acts as IL-6 agonist and involves in JAK/STAT, MAPK, and Akt signaling pathway[1][2]. IL-6R alpha/CD126, Human consists of 468 amino acids (M1-R468) with two fibronectin type-III-like domains contained in the N-terminal part (113-217 a.a, 218-316 a.a), and a soluble form (1-365 a.a). Soluble IL-6R (sIL-6R) binds IL-6 and dimerized gp130 to achieve trans signaling, and exhibits a tissue expression property in some immune systems. IL-6R alpha Protein, Human (M1-P365) is soluble form and produced in HEK293 cells with a C-Terminal hFc-tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 4.366 ng/mL in the presence of 2 ng/mL recombinant mouse IL-6, corresponding to a specific activity is 2.29×10^5 units/mg. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 4.366 ng/mL in the presence of 2 ng/mL recombinant mouse IL-6, corresponding to a specific activity is 2.29×105 units/mg.
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Alternative Names
Interleukin-6 receptor subunit alpha; IL-6R-alpha; IL-6R 1; gp80; CD126
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
P08887
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Expression Region
L20-P365
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AA Sequence
LAPRRCPAQEVARGVLTSLPGDSVTLTCPGVEPEDNATVHWVLRKPAAGSHPSRWAGMGRRLLLRSVQLHDSGNYSCYRAGRPAGTVHLLVDVPPEEPQLSCFRKSPLSNVVCEWGPRSTPSLTTKAVLLVRKFQNSPAEDFQEPCQYSQESQKFSCQLAVPEGDSSFYIVSMCVASSVGSKFSKTQTFQGCGILQPDPPANITVTAVARNPRWLSVTWQDPHSWNSSFYRLRFELRYRAERSKTFTTWMVKDLQHHCVIHDAWSGLRHVVQLRAQEEFGQGEWSEWSPEAMGTPWTESRSPPAENEVSTPMQALTTNKDDDNILFRDSANATSLPVQDSSSVPLP
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Protein Length
Extracellular Domain
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Molecular Weight
80-100 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
Interleukin-6 receptor alpha (IL-6RA) plays a pivotal role in the IL-6 signaling pathway, which is crucial for various physiological and pathological processes, including inflammation, immune response, and hematopoiesis. Elevated levels of IL-6 and its receptor have been implicated in several diseases, particularly autoimmune disorders, chronic inflammatory conditions, and even cancers. Consequently, IL-6RA has garnered significant attention as a therapeutic target for drug development. The research into recombinant IL-6RA proteins serves multiple purposes: they can be utilized in basic science to elucidate IL-6 signal transduction mechanisms, to develop novel therapeutic agents or as biomarkers for disease progression and treatment response. Additionally, recombinant IL-6RA proteins can be employed in preclinical and clinical studies to explore their potential in modulating the inflammatory response. By inhibiting IL-6 signaling, these engineered proteins may provide relief in patients with hyper-inflammatory conditions. Given the growing understanding of the role of IL-6 and its receptor in various diseases, research into IL-6RA recombinant proteins not only contributes to a deeper understanding of its biological function but also heralds advances in therapeutic strategies aimed at mitigating the detrimental effects of excessive IL-6 signaling. This research has broad implications for the treatment of diverse conditions, including rheumatoid arthritis, psoriasis, and other chronic inflammatory disorders, making it a highly relevant area within the fields of immunology and pharmacology.
