Analytical Data
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Gene name
ERI1
- Application
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Alternative Names
ERI1；3'EXO；THEX1；3'-5' exoribonuclease 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IV48
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Expression Region
1-349aa
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AA Sequence
MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASDFSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESNFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINTQLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKYKYSLLTDGSWDMSKFLNIQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSKNIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK
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Molecular Weight
40 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
ERI1 (Exoribonuclease 1) is an essential enzyme involved in RNA metabolism, particularly in the degradation of small RNA molecules. Its function has been linked to various cellular processes, including the regulation of gene expression and the processing of microRNAs. Research on ERI1 has gained momentum due to its potential implications in cancer biology and other diseases whereby aberrant RNA processing is often observed. The enzyme is known to play a crucial role in maintaining the balance of RNA species within the cell, and disruptions in its activity can lead to the accumulation of harmful RNA fragments. To better understand its mechanisms and interactions, scientists have developed recombinant ERI1 proteins, enabling detailed studies of its biochemical properties and regulatory functions. These recombinant proteins are invaluable tools for investigating the enzyme's role in RNA decay pathways and provide a foundation for exploring therapeutic strategies that target ERI1's activity. Through various expression systems and purification techniques, researchers aim to elucidate the structural and functional aspects of ERI1, enhancing our understanding of RNA metabolism and its contributions to cellular homeostasis. This research not only offers insights into ERI1's role in normal cellular functions but also expands the knowledge of its involvement in disease states, potentially leading to the development of novel diagnostic and therapeutic approaches.
