Analytical Data
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Gene name
T273A
- Application
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Alternative Names
T273A；
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P04133
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Expression Region
133-324aa(T273A)
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AA Sequence
CEWDEAATALEINLEEEDDDNEDEVDEQAEQQKTHVFGQAPYSGINITKEGIQIGVEGQTPKYADKTFQPEPQIGESQWYETEINHAAGRVLKKTTPMKPCYGSYAKPTNENGGQGILVKQQNGKLESQVEMQFFSTTEAAAGNGDNLTPKVVLYSEDVDIETPDTHISYMPTIKEGNSRELMGQQSMPNRP
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Molecular Weight
50.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
T273A is a recombinant protein that has garnered significant interest in the field of molecular biology and biochemistry due to its potential applications in understanding protein interactions and enzyme mechanisms. The T273A variant refers to the site-directed mutagenesis at threonine 273, which alters the protein's native structure and function. Researchers are particularly focused on this mutation as it may influence the protein's stability, activity, and binding affinity with substrates or inhibitors. Understanding the conformation changes induced by such mutations is crucial for elucidating the biochemical pathways that the protein participates in. Furthermore, T273A serves as a valuable tool for probing the structural dynamics of related proteins, potentially leading to insights that can be applied in drug design and development. By analyzing the functional consequences of this specific mutation, scientists aim to gain a deeper understanding of the underlying mechanisms that drive cellular processes and disease states, ultimately contributing to therapeutic advancements. The study of T273A and comparable recombinant proteins is pivotal for advancing the field of structural biology and enhancing our comprehension of complex biological systems.
