Analytical Data
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Gene name
CXCR2
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简介
CXCR2, the receptor for interleukin-8 (IL-8), orchestrates neutrophil activation through a G-protein-mediated phosphatidylinositol-calcium second messenger system upon IL-8 binding. Exhibiting high-affinity binding to IL-8, CXCR2 also interacts with other ligands like CXCL3, GRO/MGSA, and NAP-2. The involvement of GNAI2 underscores the intricate signaling mechanisms regulating neutrophil function through CXCR2. CXCR2 Protein, Human (His-SUMO) is the recombinant human-derived CXCR2 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag.
- Application
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Alternative Names
C-X-C motif chemokine receptor 2; IL8RB, interleukin 8 receptor, beta; C-X-C chemokine receptor type 2; CD182; CMKAR2;
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Species
Human
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Source
E. coli
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Tag
N-SUMO;N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P25025
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Expression Region
M1-E40
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Protein Length
Partial
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Molecular Weight
23-25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CXCR2, a member of the CXC chemokine receptor family, is a G-protein coupled receptor that plays a pivotal role in regulating immune responses, inflammation, and hematopoiesis by mediating the recruitment of neutrophils to sites of infection or injury. Its ligands, including IL-8 and other CXC chemokines, bind to CXCR2 and activate downstream signaling pathways that influence various cellular functions such as migration, survival, and activation of immune cells. Dysregulation of CXCR2 signaling has been implicated in several pathological conditions, including cancer progression, chronic inflammatory diseases, and respiratory disorders. As a result, CXCR2 has emerged as a potential therapeutic target. Researchers have been increasingly focused on generating CXCR2 recombinant proteins to study its structure-function relationships, binding properties, and interactions with specific ligands. The production of these recombinant proteins allows for detailed functional analyses and the development of CXCR2 antagonists or agonists. Additionally, understanding the receptor’s signaling mechanisms and its role in disease contexts can provide insights for novel therapeutic strategies, thereby highlighting the importance of CXCR2 in both basic research and clinical applications.
