Analytical Data
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Gene name
TNFa
- Application
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Alternative Names
TNF-a; TNF-alpha; TNFA; TNFA_HUMAN; TNFSF2
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Species
Human
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Source
E. coli
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Tag
N-terminal His Tag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P01375
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Expression Region
77-233aa
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AA Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIK SPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
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Molecular Weight
21.0kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Identification
Protein Description
TNF-alpha induced protein 3 (TNFAIP3), also known as A20, is a key regulator of inflammatory responses and immune signaling pathways. It is crucial for maintaining cellular homeostasis and has been implicated in various pathologies, including autoimmune diseases, inflammatory disorders, and cancer. TNFAIP3 functions primarily as an inhibitor of NF-kB signaling, which plays a pivotal role in inflammation and cell survival. Dysregulation of TNFAIP3 expression or activity can lead to uncontrolled inflammatory responses and is associated with conditions such as rheumatoid arthritis, systemic lupus erythematosus, and certain malignancies. The study of recombinant TNFAIP3 is essential to unravel its biological functions and regulatory mechanisms. By producing this protein in a recombinant form, researchers can explore its molecular interactions and therapeutic potential. Understanding TNFAIP3's structure-function relationship may facilitate the development of novel strategies for manipulating inflammatory pathways and designing targeted therapies for diseases characterized by TNFAIP3 dysfunction. Consequently, ongoing research focuses on optimizing recombinant protein production techniques and elucidating the role of TNFAIP3 in various cellular contexts to advance our understanding of immune regulation and disease mechanisms.
