Analytical Data
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Gene name
NBC1
- Application
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Alternative Names
SLC4A4;NBC;NBC1;NBCE1;Electrogenic sodium bicarbonate cotransporter 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6R1
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Expression Region
1-580aa
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AA Sequence
MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKTGHKEKKEKERISENYSDKSDIENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIVDHQIETGLLKPELKDKVTYTLLRKHRHQTKKSNLRSLADIGKTVSSASRMFTNPDNGSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKAKDRHDLIAGIDEFLDEVIVLPPGEWDPAIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDNNFDYLEFRLWIGLWSAFLCLILVATDASFLVQYF
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NBC1 (SLC4A4) is a member of the solute carrier family and functions as a sodium-bicarbonate cotransporter, playing a crucial role in maintaining acid-base balance in various tissues, particularly in the kidneys and the central nervous system. The study of NBC1 recombinant protein has gained significant interest due to its involvement in physiological processes linked to pH regulation and bicarbonate transport, which are vital in conditions such as metabolic acidosis and certain neurological disorders. Research indicates that alterations in NBC1 expression or function can lead to disorders like proximal renal tubular acidosis and may influence neurodegenerative conditions. Understanding the structural and functional properties of NBC1 through recombinant techniques provides insights into its mechanism of action and potential therapeutic targets. Advancements in this area could pave the way for novel treatments for acid-base balance disorders and offer a deeper understanding of NBC1's role in cellular physiology and pathophysiology. The generation of NBC1 recombinant protein allows for detailed biochemical assays, characterization, and potential drug screening, thus making it a focal point of research aimed at addressing unmet clinical needs related to bicarbonate transport and associated diseases.
