Analytical Data
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Gene name
cysK
- Application
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Alternative Names
cysK；Cysteine synthase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A535
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Expression Region
1-310aa
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AA Sequence
MSIAEDITQLIGRTPLVRLRRVTDGAVADIVAKLEFFNPANSVKDRIGVAMLQAAEQAGLIKPDTIILEPTSGNTGIALAMVCAARGYRCVLTMPETMSLERRMLLRAYGAELILTPGADGMSGAIAKAEELAKTDQRYFVPQQFENPANPAIHRVTTAEEVWRDTDGKVDIVVAGVGTGGTITGVAQVIKERKPSARFVAVEPAASPVLSGGQKGPHPIQGIGAGFVPPVLDQDLVDEIITVGNEDALNVARRLAREEGLLVGISSGAATVAALQVARRPENAGKLIVVVLPDFGERYLSTPLFADVAD
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Molecular Weight
40.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CysK, or cysteine synthase, plays a crucial role in the biosynthesis of cysteine, an essential amino acid that serves as a building block for proteins and is involved in various metabolic processes. This enzyme catalyzes the conversion of O-phosphoserine and hydrogen sulfide to cysteine, a reaction that is pivotal for cellular functions, including antioxidant defense and detoxification mechanisms. Research on CysK has gained significance due to its potential implications in various health conditions, such as neurodegenerative diseases, where cysteine levels are often disrupted. Moreover, CysK is of interest in biotechnology and pharmaceutical industries, particularly for the production of cysteine and its derivatives. Recombinant protein studies of CysK provide insights into its enzymatic mechanisms, structural characteristics, and interactions with substrates and inhibitors. Understanding the properties of recombinant CysK can pave the way for developing targeted therapeutic strategies and enhancing cysteine production in industrial applications. As a result, the investigation of CysK is expected to contribute significantly to both fundamental biological research and applied sciences, highlighting its importance in metabolism and potential for therapeutic interventions.
