Analytical Data
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Gene name
LONP1
- Application
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Alternative Names
LONP1；PRSS15；Lon protease homolog. mitochondrial
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P36776
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Expression Region
124-368aa
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AA Sequence
LPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKK
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Molecular Weight
35.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
LONP1, or Lon protease 1, is a mitochondrial matrix protein with critical roles in protein quality control and mitochondrial homeostasis. It is an essential ATP-dependent protease that regulates mitochondrial protein levels, helping to prevent the accumulation of misfolded or damaged proteins, which can lead to cellular dysfunction and various diseases. The study of LONP1 has gained importance due to its implications in cancer, neurodegenerative disorders, and metabolic diseases, highlighting its potential as a therapeutic target. Research has shown that altered LONP1 expression or activity can influence cellular stress responses, impact apoptosis, and modulate the production of reactive oxygen species. Furthermore, LONP1 interacts with multiple substrates, suggesting that it plays a broader role in mitochondrial function beyond just degradation. Given the increasing recognition of mitochondrial dysfunction in various pathologies, understanding LONP1's mechanistic roles and regulatory pathways is vital. Recent advances in recombinant protein technology have enabled detailed studies on LONP1 activity, structure, and interactions, paving the way for potential therapeutic interventions. The exploration of LONP1 as a research focus could unravel new insights into mitochondrial dynamics and contribute to the development of novel strategies for treating mitochondrial-related diseases.
