Analytical Data
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Gene name
KRT15
- Application
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Alternative Names
AI528832; CK 15; CK-15; CK15; Cytokeratin-15; Cytokeratin15; K15; K1C15_HUMAN; K1CO; Ka15; Keratin 15 ; Keratin 15 basic; Keratin 15 beta; Keratin; Keratin complex 1 acidic gene 15; Keratin type I cytoskeletal 15; Keratin-15; Keratin15; KRT 15; Krt1-15; KRT15; KRTB; KRTL15; Type I cytoskeletal 15; Type I keratin Ka15
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P19012
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Expression Region
1-456aa
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AA Sequence
MTTTFLQTSSSTFGGGSTRGGSLLAGGGGFGGGSLSGGGGSRSISASSARFVSSGSGGGYGGGMRVCGFGGGAGSVFGGGFGGGVGGGFGGGFGGGDGGLLSGNEKITMQNLNDRLASYLDKVRALEEANADLEVKIHDWYQKQTPTSPECDYSQYFKTIEELRDKIMATTIDNSRVILEIDNARLAADDFRLKYENELALRQGVEADINGLRRVLDELTLARTDLEMQIEGLNEELAYLKKNHEEEMKEFSSQLAGQVNVEMDAAPGVDLTRVLAEMREQYEAMAEKNRRDVEAWFFSKTEELNKEVASNTEMIQTSKTEITDLRRTMQELEIELQSQLSMKAGLENSLAETECRYATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIGIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI
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Molecular Weight
75.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KRT15, also known as Keratin 15, is a type I keratin expressed predominantly in the basal layer of the epidermis and hair follicles. It plays a crucial role in maintaining the structural integrity and function of epithelial tissues. Research into KRT15 has gained prominence due to its involvement in various skin disorders, such as epidermolysis bullosa and psoriasis, as well as its potential role in hair follicle biology and development. The study of recombinant KRT15 proteins has become essential for elucidating the molecular mechanisms underlying these conditions. By generating and characterizing recombinant KRT15, researchers aim to better understand its oligomerization properties, interaction with other cytoskeletal proteins, and its regulatory pathways during skin and hair follicle differentiation. Furthermore, recombinant KRT15 proteins serve as valuable tools in investigating the protein's functional attributes and possibilities for therapeutic applications, including regenerative medicine and gene therapy. Overall, research on KRT15 and its recombinant forms enhances our knowledge of keratin biology and its implications in dermatological health and disease.
