Analytical Data
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Gene name
KNTC2
- Application
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Alternative Names
from Entrez Gene .; HEC; Highly expressed in cancer; Highly expressed in cancer protein; Highly expressed in cancer rich in leucine heptad repeats; HsHec1; hsNDC80; Kinetochore associated 2; Kinetochore associated protein 2; Kinetochore protein Hec1; Kinetochore protein NDC80 homolog; Kinetochore-associated protein 2; KNTC2; ndc80; NDC80 homolog kinetochore complex component
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14777
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Expression Region
1-642aa
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AA Sequence
MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS ERKVSLFGKR TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC EFLTENGYAH NVSMKSLQAP SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE VPRIFKDLGY PFALSKSSMY TVGAPHTWPH IVAALVWLID CIKIHTAMKE SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA DSFDEMNAEL QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT RLQNIIDNQK YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK YARGKEAIET QLAEYHKLAR KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRAQVYVP LKELLNETEE EINKALNKKM GLEDTLEQLN AMITESKRSV RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH LLESTVNQGL SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE
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Molecular Weight
73.9 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KNTC2, a member of the Kinesin-14 family, plays a crucial role in various cellular processes, including mitosis, intracellular transport, and the organization of the cytoskeleton. Its significance has been underscored by emerging evidence linking KNTC2 to essential functions in chromosome alignment and spindle organization during cell division. The protein's unique motor domain and ability to interact with microtubules make it a pivotal player in the dynamics of the mitotic spindle, which is fundamental for maintaining genomic stability. Dysfunction or dysregulation of KNTC2 has been associated with various diseases, including cancer, where improper chromosomal segregation can lead to aneuploidy and tumorigenesis. Consequently, there is an increasing interest in studying KNTC2 through recombinant protein technologies. By expressing and purifying KNTC2 in model systems, researchers aim to elucidate its structure-function relationship and the molecular mechanisms underlying its role in mitosis and cellular transport. Investigating KNTC2 using recombinant proteins also facilitates the development of potential therapeutic interventions targeting its pathways in diseases characterized by cell division abnormalities. Overall, the study of KNTC2 recombinant protein is essential for advancing our understanding of cell biology and may provide novel insights into targeted treatments for cancer and other related disorders.
