Analytical Data
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基因名
LTA4H
- Application
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别名
LTA4H;LTA4;Leukotriene A-4 hydrolase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P09960
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表达区间
1-611aa
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氨基酸序列
MPEIVDTCSLASPASVCRTKHLHLRCSVDFTRRTLTGTAALTVQSQEDNL RSLVLDTKDLTIEKVVINGQEVKYALGERQSYKGSPMEISLPIALSKNQE IVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQCQAIHCRAILPCQDT PSVKLTYTAEVSVPKELVALMSAIRDGETPDPEDPSRKIYKFIQKVPIPC YLIALVVGALESRQIGPRTLVWSEKEQVEKSAYEFSETESMLKIAEDLGG PYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEISH SWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGEL QNSVKTFGETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGG PEIFLGFLKAYVEKFSYKSITTDDWKDFLYSYFKDKVDVLNQVDWNAWLY SPGLPPIKPNYDMTLTNACIALSQRWITAKEDDLNSFNATDLKDLSSHQL NEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFRWLRLCIQSKWED AIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQAVRTYQEHKASMHPVT AMLVGKDLKVDDYKDDDDKHHHHHH
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分子量
71 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Lipid mediator molecules play a crucial role in regulating various physiological processes, and LTA4H (leukotriene A4 hydrolase) is a pivotal enzyme involved in the biosynthesis of leukotrienes, which are lipid compounds that mediate inflammatory responses. LTA4H catalyzes the conversion of leukotriene A4 into leukotriene B4, a potent chemotactic agent that attracts immune cells to sites of inflammation. Dysfunction or dysregulation of LTA4H has been implicated in various inflammatory diseases, including asthma, rheumatoid arthritis, and cardiovascular diseases. Recent studies have highlighted the potential of LTA4H as a therapeutic target, given its critical role in leukotriene metabolism. Research on recombinant LTA4H proteins has advanced our understanding of its enzymatic mechanisms and regulatory pathways, necessitating the expression and purification of these proteins for structural and functional studies. This recombinant approach not only facilitates the investigation of LTA4H’s role in pathophysiology but also aids in the development of selective inhibitors that can modulate its activity for therapeutic benefits. Through these studies, scientists aim to elucidate the detailed mechanisms of LTA4H and its contributions to inflammation, ultimately paving the way for novel interventions in inflammatory diseases.
