Analytical Data
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Gene name
KIF1B
- Application
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Alternative Names
KIF1B; KIAA0591; KIAA1448Kinesin-like protein KIF1B; Klp
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60333
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Expression Region
1-579aa
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AA Sequence
MSKTSLGQSMSKYDLLVWFEISELEPTGEYIPAVVDHTAGLPCQGTFLLHQGIQRRITVTIIHEKGSELHWKDVRELVVGRIRNKPEVDEAAVDAILSLNIISAKYLKSSHNSSRTFYRFEAVWDSSLHNSLLLNRVTPYGEKIYMTLSAYLELDHCIQPAVITKDVCMVFYSRDAKISPPRSLRSLFGSGYSKSPDSNRVTGIYELSLCKMSDTGSPGMQRRRRKILDTSVAYVRGEENLAGWRPRGDSLILEHQWELEKLELLHEVEKTRHFLLLRERLGDSIPKSLSDSLSPSLSSGTLSTSTSISSQISTTTFESAITPSESSGYDSGDIESLVDREKELATKCLQLLTHTFNREFSQVHGSVSDCKLSDISPIGRDPSESSFSSATLTPSSTCPSLVDSRSNSLDQKTPEANSRASSPCPEFEQFQIVPAVETPYLARAGKNEFLNLVPDIEEIRPSSVVSKKGYLHFKEPLYSNWAKHFVVVRRPYVFIYNSDKDPVERGIINLSTAQVEYSEDQQAMVKTPNTFAVCTKHRGVLLQALNDKDMNDWLYAFNPLLAGTIRSKLSRRCPSQSKY
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Molecular Weight
89.21 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
KIF1B, a member of the kinesin superfamily of motor proteins, has garnered significant attention due to its pivotal role in cellular transport mechanisms, particularly in the anterograde transportation of organelles and vesicles along microtubules. Research has demonstrated that KIF1B is crucial for neuronal development and function, as it facilitates the transport of critical cargoes such as synaptic vesicles and signaling molecules in axons and dendrites. Moreover, emerging studies have associated KIF1B with various pathological conditions, including neurodegenerative diseases and certain types of cancer, where its malfunction can disrupt cellular homeostasis and lead to disease progression. Understanding the structure and function of KIF1B is essential for elucidating its mechanisms of action and potential therapeutic targets. Recombinant KIF1B proteins have been produced to explore their biochemical properties and interactions in vitro, allowing researchers to investigate the motor protein's activity, binding sites, and regulatory mechanisms. These studies are crucial for developing strategies aimed at modulating KIF1B activity for therapeutic benefit in diseases related to its dysfunction. Overall, KIF1B represents a compelling focus within molecular and cellular biology, offering insights into fundamental transport processes that are critical for maintaining cellular integrity and function.
