Cat: PA2000-4579

Recombinant Human CATSPER1 Protein,His

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Analytical Data

  • Gene name

    CATSPER1

  • Application

    SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

  • Alternative Names

    CATSPER1;Cation channel sperm-associated Protein 1

  • Species

    Human

  • Source

    E. coli

  • Tag

    His tag N-Terminus

  • Purity

    Greater than 90% as determined by SDS-PAGE.

  • Uniprot

    Q8NEC5

  • Expression Region

    1-445aa

  • AA Sequence

    MDQNSVPEKAQNEADTNNADRFFRSHSSPPHHRPGHSRALHHYELHHHGVPHQRGESHHPPEFQDFHDQALSSHVHQSHHHSEARNHGRAHGPTGFGLAPSQGAVPSHRSYGEDYHDELQRDGRRHHDGSQYGGFHQQSDSHYHRGSHHGRPQYLGENLSHYSSGVPHHGEASHHGGSYLPHGPNPYSESFHHSEASHLSGLQHDESQHHQVPHRGWPHHHQVHHHGRSRHHEAHQHGKSPHHGETISPHSSVGSYQRGISDYHSEYHQGDHHPSEYHHGDHPHHTQHHYHQTHRHRDYHQHQDHHGAYHSSYLHGDYVQSTSQLSIPHTSRSLIHDAPGPAASRTGVFPYHVAHPRGSAHSMTRSSSTIRSRVTQMSKKVHTQDISTKHSEDWGKEEGQFQKRKTGRLQRTRKKGHSTNLFQWLWEKLTFLIQGFREMIRNLTQ

  • Molecular Weight

    52.7 kDa

  • Endotoxin

    < 1.0 EU per μg protein as determined by the LAL method.

  • Form

    Freeze-dried powder

  • Buffer formulation

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • Reconstitution

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • Customization

    Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

  • Stability Test

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • Storage & Shelf Life

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • Shipping

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

CATSPER1 (Cation Channel Sperm Associated 1) is a crucial protein involved in sperm motility and fertilization. It is a member of the CatSper channel family, which plays a significant role in the calcium influx necessary for the activation and forward motility of sperm. Deficiencies or mutations in CATSPER1 are linked to male infertility, making it an important target for reproductive health research. Understanding the structure and function of CATSPER1 can provide insights into its role in sperm physiology and the mechanisms underlying sperm motility and acrosome reaction during fertilization. The recombinant expression of CATSPER1 allows for detailed biochemical and biophysical studies, enabling researchers to investigate its interactions with other proteins and ion channels. Furthermore, insights gained from studying CATSPER1 can have potential implications for the development of contraceptives and treatments for male infertility. By characterizing this protein through recombinant techniques, researchers aim to elucidate its functional mechanisms and explore new therapeutic avenues in reproductive medicine. Overall, the study of CATSPER1 represents a vital area of research in understanding male reproductive health and addressing infertility challenges.

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