Analytical Data
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Gene name
IKBKE
- Application
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Alternative Names
I kappa B kinase epsilon; I-kappa-B kinase epsilon; IkBKE; IKK related kinase epsilon; IKK-E; IKK-epsilon; IKK-i; IKKE; IKKE_HUMAN; IKKepsilon; IKKI; Inducible I kappa B kinase; Inducible I kappa-B kinase; Inducible IkappaB kinase; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon; Inhibitor of kappa light polypeptide gene enhancer in B cells. kinase of
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q14164
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Expression Region
1-716aa
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AA Sequence
MQSTANYLWH TDDLLGQGAT ASVYKARNKK SGELVAVKVF NTTSYLRPRE VQVREFEVLR KLNHQNIVKL FAVEETGGSR QKVLVMEYCS SGSLLSVLES PENAFGLPED EFLVVLRCVV AGMNHLRENG IVHRDIKPGN IMRLVGEEGQ SIYKLTDFGA ARELDDDEKF VSVYGTEEYL HPDMYERAVL RKPQQKAFGV TVDLWSIGVT LYHAATGSLP FIPFGGPRRN KEIMYRITTE KPAGAIAGAQ RRENGPLEWS YTLPITCQLS LGLQSQLVPI LANILEVEQA KCWGFDQFFA ETSDILQRVV VHVFSLSQAV LHHIYIHAHN TIAIFQEAVH KQTSVAPRHQ EYLFEGHLCV LEPSVSAQHI AHTTASSPLT LFSTAIPKGL AFRDPALDVP KFVPKVDLQA DYNTAKGVLG AGYQALRLAR ALLDGQELMF RGLHWVMEVL QATCRRTLEV ARTSLLYLSS SLGTERFSSV AGTPEIQELK AAAELRSRLR TLAEVLSRCS QNITETQESL SSLNRELVKS RDQVHEDRSI QQIQCCLDKM NFIYKQFKKS RMRPGLGYNE EQIHKLDKVN FSHLAKRLLQ VFQEECVQKY QASLVTHGKR MRVVHETRNH LRLVGCSVAA CNTEAQGVQE SLSKLLEELS HQLLQDRAKG AQASPPPIAP YPSPTRKDLL LHMQELCEGM KLLASDLLDN NRIIERLNRV PAPPDV
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Molecular Weight
80.4 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
IKBKE (Inhibitor of Nuclear Factor Kappa B Kinase Epsilon) is a member of the IKK family and plays a critical role in the regulation of the NF-κB signaling pathway, which is essential for various cellular processes, including immune response, inflammation, and cell survival. The dysregulation of IKBKE has been linked to several diseases, including cancer and autoimmune disorders, making it a significant target for therapeutic interventions. Recent studies have highlighted IKBKE’s involvement in the phosphorylation and activation of NF-κB, leading to the expression of a range of pro-inflammatory cytokines and survival factors. Furthermore, research has revealed that IKBKE can have distinct functions depending on cellular context, influencing not only immune cells but also tumor microenvironments. As a result, the development of IKBKE recombinant proteins is gaining attention for exploring its functional roles and potential as a biomarker or therapeutic target. Investigating the structure and function of IKBKE through recombinant protein techniques can provide insights into its regulatory mechanisms and its interactions within the NF-κB pathway. The characterization of IKBKE could pave the way for novel approaches in disease treatment, particularly in designing specific inhibitors that can modulate IKBKE activity to restore normal signaling pathways. Overall, the study of IKBKE recombinant proteins is poised to enhance our understanding of its biological significance and therapeutic potential in various pathophysiological conditions.
